The cytokine response of afferent lymph following orf virus reinfection of sheep
The cytokine response of afferent lymph following orf virus reinfection of sheep
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DOI：
10.1111/j.1365-3164.1996.tb00221.x
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发表时间：
1996-03-01
影响因子：
1.4
通讯作者：
Reid, HW
中科院分区：
文献类型：
--
作者：
Haig, D;Deane, D;Reid, HW
比较了ORF病毒再感染和灭活病毒注射后，绵羊体内分化细胞动态及传入淋巴中IL-1β、IL-8、粒细胞-巨噬细胞集落刺激因子(GM-CSF)和干扰素-γ滴度的变化。淋巴中的淋巴母细胞和细胞因子对病毒再感染的双相反应与最初对ORF病毒作为召回抗原的反应是一致的，随后是对病毒复制的反应。两组淋巴中CD4T细胞的产量均高于其他类型的细胞。在病毒再次感染或注射后24小时内，随着细胞因子滴度的增加，淋巴血浆中检测到快速的免疫/炎症反应。在两组中，产生IL-Iβ和IL-8的淋巴细胞先于产生GM-CSF的淋巴细胞。尽管再感染后淋巴中单个细胞因子的浓度不同，但所有病例的ORF病变的大小和消失的时间都是相似的。
The in vivo dynamics of differentiated cells and interleukin (IL)-1 beta, IL-8, granulocyte-macrophage colony-stimulating factor (GM-CSF) and interferon (IFN)-gamma titres in afferent lymph were compared following orf virus reinfection and inactivated virus injection of previously infected sheep. The biphasic lymphoblast and cytokine response in the lymph to virus reinfection is consistent with a response initially to orf virus as recall antigen followed by a response to viral replication. CD4 T cells increased in output over other cell types in the lymph in both groups. A rapid immune/inflammatory response was detected in lymph plasma as an increase in cytokine titres within 24 h of virus reinfection or injection. Lymph cells producing IL-I beta and IL-8 appeared prior to those producing GM-CSF in both groups. In spite of variations in the concentration of individual cytokines in lymph following reinfection, both the size of the orf lesion and the time to resolve were similar in all cases.