The role of Paraxial Protocadherin in Xenopus otic placode development
The role of Paraxial Protocadherin in Xenopus otic placode development
复制标题
近轴原钙粘蛋白在爪蟾耳基板发育中的作用。
DOI：
10.1016/j.bbrc.2006.04.068
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发表时间：
2006-06-23
影响因子：
3.1
通讯作者：
Ding, Xiaoyan
中科院分区：
文献类型：
--
作者：
Hu, Rui-Ying;Xu, Peng;Ding, Xiaoyan
脊椎动物的内耳从其雏形——耳基板发展而来，随后形成耳泡并产生构成整个内耳的组织。尽管几种信号分子已被确定为负责内耳规范和模式的候选分子，但许多细节仍然难以捉摸。在这里，我们报告近轴原钙粘蛋白（PAPC）是非洲爪蟾胚胎中耳囊形成所必需的。 PAPC 在内耳形态发生过程中严格在内耳基板中表达，随后在耳囊中表达。显性失活 PAPC 敲低 PAPC 导致耳囊泡形成失败以及早期内耳标志物 Sox9 和 Tbx2 的丢失，表明 PAPC 在耳囊发育的早期阶段是必需的。然而，单独的 PAPC 不足以诱导耳基板形成。 (c) 2006 Elsevier Inc. 保留所有权利。
Vertebrate inner ear develops from its rudiment, otic placode, which later forms otic vesicle and gives rise to tissues comprising the 14 entire inner ear. Although several signaling molecules have been identified as candidates responsible for inner ear specification and patterning, many details remain elusive. Here, we report that Paraxial Protocadherin (PAPC) is required for otic vesicle formation in Xenopus embryos. PAPC is expressed strictly in presumptive otic placode and later in otic vesicle during inner ear morphogenesis. Knockdown of PAPC by dominant-negative PAPC results in the failure of otic vesicle formation and the loss of early inner ear markers Sox9 and Tbx2, suggesting the requirement of PAPC in the early stage of otic vesicle development. However, PAPC alone is not sufficient to induce otic placode formation. (c) 2006 Elsevier Inc. All rights reserved.