Tuning the dynamics and molecular distribution of the self-spreading lipid bilayer.
Tuning the dynamics and molecular distribution of the self-spreading lipid bilayer.
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DOI：
10.1039/b715983h
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发表时间：
2008-04
期刊：
影响因子：
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通讯作者：
H. Nabika;Akihisa Fukasawa;K. Murakoshi
中科院分区：
文献类型：
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作者：
H. Nabika;Akihisa Fukasawa;K. Murakoshi
在控制电解质浓度的脂质双层的自扩展动力学进行了研究。当NaCl浓度从1增加到100 mM时，自铺展速度增加。将实验测定的铺展能与理论模型估算的铺展能进行比较，我们发现考虑自铺展双层与基底之间的货车德瓦尔斯相互作用能、双层相互作用能和水合相互作用能，可以很好地解释自铺展动力学。高浓度下的特征行为归因于脂质层密度的增加，这源于溶液中电解质离子对分子电荷的有效屏蔽。掺杂染料标记分子在扩散双层内的分布也可以通过调节电解质浓度来控制。所有这些发现被解释的双层基板或分子间的相互作用取决于电解质浓度的系统变化。
The self-spreading dynamics of lipid bilayers were investigated at controlled electrolyte concentrations. The self-spreading velocity increased when the concentration of NaCl was increased from 1 to 100 mM. Comparing the experimentally determined spreading energy with that estimated from theoretical models, we found that the self-spreading dynamics were well explained by considering the van der Waals interaction, double layer interaction and hydration interaction energies between the self-spreading bilayer and the substrate. The characteristic behavior at high concentration is attributable to the increase in the density of the lipid layer, originating from the effective shielding of the molecular charges by the electrolyte ions in solution. The distribution of doped dye-labeled molecule within the spreading bilayer was also controllable by tuning the electrolyte concentration. All of these findings were explained by systematic changes in bilayer-substrate or inter-molecular interactions depending on the electrolyte concentration.