Complete genome sequence of Montana Myotis leukoencephalitis virus, phylogenetic analysis and comparative study of the 3′ untranslated region of flaviviruses with no known vector
Complete genome sequence of Montana Myotis leukoencephalitis virus, phylogenetic analysis and comparative study of the 3′ untranslated region of flaviviruses with no known vector
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DOI：
10.1099/0022-1317-83-8-1875
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发表时间：
2002-08-01
影响因子：
3.8
通讯作者：
Neyts, J
中科院分区：
文献类型：
--
作者：
Charlier, N;Leyssen, P;Neyts, J
蒙大拿州肌炎白质脑炎病毒(MMLV)是一种从蝙蝠中分离出来的病毒，它会引起小型啮齿动物的脑炎，使人想起人类的黄病毒脑炎。MMLV基因组全长10690个核苷酸，编码3374个氨基酸的多聚蛋白。该病毒在被认为是有趣的抗病毒靶标(NTPase/解旋酶、丝氨酸蛋白酶和RNA依赖的RNA聚合酶)的基因中包含与具有临床重要性的黄病毒相同的保守基序。对整个编码区的系统发育分析证实了MMLV在未知载体的黄病毒分支(NKV)中的分类，并在这个分支中归入Rio Bravo分支(两种病毒都以蝙蝠为脊椎动物宿主)。我们首次对NKV黄病毒(除MMLV外，还有Modoc病毒、Rio Bravo病毒和Apoi病毒)的3‘UTR的RNA折叠进行了比较分析。已发现保存在其他黄病毒中的3‘非编码区结构元素，以及将NKV与蚊媒和扁虱传播的黄病毒区分开来的元素。特别是，在所有蚊媒和扁虱传播的黄病毒中保守的五核苷酸序列5‘CACAG 3’在所有四种NKV黄病毒的3‘长稳定发夹结构的环中被5’C(C/U)(C/U)AG 3‘序列取代。后一个序列基序的可用性使我们能够将病毒指定为NKV或媒介传播的黄病毒。
Montana Myotis leukoencephalitis virus (MMLV), a virus isolated from bats, causes an encephalitis in small rodents reminiscent of flavivirus encephalitis in humans. The complete MMLV genome is 10690 nucleotides long and encodes a putative polyprotein of 3374 amino acids. The virus contains the same conserved motifs in genes that are believed to be interesting antiviral targets (NTPase/helicase, serine protease and RNA-dependent RNA polymerase) as flaviviruses of clinical importance. Phylogenetic analysis of the entire coding region has confirmed the classification of MMLV in the clade of the flaviviruses with no known vector (NKV) and within this clade to the Rio Bravo branch (both viruses have the bat as their vertebrate host). We have provided for the first time a comparative analysis of the RNA folding of the 3' UTR of the NKV flaviviruses (Modoc, Rio Bravo and Apoi viruses, in addition to MMLV). Structural elements in the 3' UTR that are preserved among other flaviviruses have been revealed, as well as elements that distinguish the NKV from the mosquito- and tick-borne flaviviruses. In particular, the pentanucleotide sequence 5' CACAG 3', which is conserved in all mosquito- and tick-borne flaviviruses, is replaced by the sequence 5' C(C/U)(C/U)AG 3' in the loop of the 3' long stable hairpin structure of all four NKV flaviviruses. The availability of this latter sequence motif allows us to designate a virus as either an NKV or a vector-borne flavivirus.