Circulating lipopolysaccharide-binding protein (LBP) as a marker of obesity-related insulin resistance
Circulating lipopolysaccharide-binding protein (LBP) as a marker of obesity-related insulin resistance
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DOI：
10.1038/ijo.2011.256
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发表时间：
2012-11-01
影响因子：
4.9
通讯作者：
Fernandez-Real, J. M.
中科院分区：
文献类型：
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作者：
Moreno-Navarrete, J. M.;Ortega, F.;Fernandez-Real, J. M.
目的：脂多糖结合蛋白（Lipopolysaccharide-binding protein，LBP）是一种高浓度存在于血液中的65 kDa急性期蛋白，已知来源于肝脏。我们的目的是深入了解循环LBP与人类和小鼠胰岛素抵抗的关系。方法，设计和测量：我们研究了LBP的横截面（n = 222）和体重减轻（n = 34）相关性（酶联免疫吸附试验）与炎症和代谢参数（包括最小模型测量的胰岛素敏感性），以及高脂饮食（HFD）、二甲双胍和遗传胰岛素致敏作用结果：循环LBP浓度在2型糖尿病受试者中显著增加，并且在病态肥胖受试者中显著增加。LBP与胰岛素敏感性和不同炎症标志物显着相关，并在体重减轻后下降（22.2 +/- 5.8 vs 16.2 +/- 9.3 μ g ml（-1），P
OBJECTIVE: Lipopolysaccharide-binding protein (LBP) is a 65-kDa acute-phase protein present in blood at high concentrations, known to be derived from the liver. We aimed to gain insights into the association of circulating LBP with insulin resistance in humans and mice.METHODS, DESIGN AND MEASUREMENTS: We studied the cross-sectional (n = 222) and weight loss-induced (n = 34) associations of LBP (enzyme-linked immunosorbent assay) with inflammatory and metabolic parameters (including minimal model-measured insulin sensitivity), and the effects of high-fat diet (HFD), metformin and genetic insulin sensitization (glucagon-like peptide 1 receptor knockout model) in mice.RESULTS: Circulating LBP concentration was significantly increased in subjects with type 2 diabetes and dramatically increased in subjects with morbid obesity. LBP was significantly associated with insulin sensitivity and different inflammatory markers and decreased after weight loss (22.2 +/- 5.8 vs 16.2 +/- 9.3 mu g ml(-1), P