Progenitor cell expansion: an important source of hepatocyte regeneration in chronic hepatitis
Progenitor cell expansion: an important source of hepatocyte regeneration in chronic hepatitis
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DOI：
10.1016/j.jhep.2004.08.017
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发表时间：
2004-12-01
影响因子：
25.7
通讯作者：
Bhagat, G
中科院分区：
文献类型：
--
作者：
Eleazar, JA;Memeo, L;Bhagat, G
背景/目的：在多种慢性肝病中已经证实了祖细胞活化并随后成熟为肝细胞和胆系细胞，但是在慢性肝炎中祖细胞反应的动力学和幅度尚未得到充分详细的研究。我们进行了这项研究，以评估负责祖细胞/小管反应的因素，并进一步剖析疾病分级和阶段作为慢性肝炎双能祖细胞肝细胞分化的决定因素的作用。对慢性丙型肝炎（n = 47）、B型肝炎（n = 20）和自身免疫性肝炎（n = 20）患者的细胞角蛋白7（和19）染色活检进行了研究。倍体分析和增殖指数进行了评估，在一个子集的cases.Results：在大多数情况下（97%），出现在疾病的早期，并与疾病的活动性，而祖细胞衍生的肝细胞出现在疾病后期，其程度与疾病阶段。所有细胞类型的增殖指数与疾病activity.Conclusions：祖细胞衍生的肝细胞在慢性肝炎中累积，可能与原生肝细胞功能障碍有关。然而，这些肝细胞的命运尚不清楚。(C)2004年欧洲肝脏研究协会。Elsevier B. V.出版，保留所有权利。
Background/Aims: Progenitor cell activation with subsequent maturation to hepatocytes and cells of the biliary lineage has been demonstrated in a variety of chronic liver diseases but the kinetics and magnitude of the progenitor cell response has not been adequately studied in detail in chronic hepatitis. We undertook this study to evaluate factors responsible for the progenitor cell/ductular response and further dissect the role of disease grade and stage as determinants of hepatocellular differentiation of bipotential progenitor cells in chronic hepatitis.Methods: Cytokeratin 7 (and 19) stained biopsies from patients with chronic hepatitis C (n = 47), hepatitis B (n = 20), and autoimmune hepatitis (n = 20) were studied. Ploidy analysis and proliferation indices were evaluated in a subset of cases.Results: Ductular reactions were present in the majority of cases (97%), appeared early in disease, and correlated with disease activity, while progenitor cell derived hepatocyes appeared later in disease and their extent correlated with disease stage. Proliferation indices of all cell types correlated with disease activity.Conclusions: Progenitor cell derived hepatocytes accrue in chronic hepatitis, possibly related to native hepatocellular dysfunction. However, the fate of these hepatocytes is unclear. (C) 2004 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.