Introduction of DNA into S. pombe cells.
Introduction of DNA into S. pombe cells.
复制标题
DOI：
10.1002/0471142727.mb1317s64
复制
发表时间：
2003-11-01
影响因子：
--
通讯作者：
Forsburg, Susan L
中科院分区：
文献类型：
--
作者：
Forsburg, Susan L
转化方法依赖于调节细胞吸收DNA，并在有选择的条件下培养它们，以建立和保持质粒或整合。可以使用不同的方法，包括电穿孔、锂离子处理或去除细胞壁的原生质体处理。为转化而准备的原生质体也可以被诱导相互融合并进行核受精，这为不育株的交配提供了一种手段。
Methods of transformation rely upon conditioning cells to take up DNA, and growing them under selective conditions to establish and maintain the plasmid or integration. Different methods may be used, including electroporation, treatment with lithium cations, or protoplast treatment which removes the cell wall. Protoplasts prepared as for transformation can also be induced to fuse with each other and undergo karyogamy, which provides a means of mating sterile strains.