Presynaptic peptide receptors and hypertension.
Presynaptic peptide receptors and hypertension.
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突触前肽受体和高血压。
DOI：
10.1111/j.1749-6632.1990.tb32006.x
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发表时间：
1990
影响因子：
5.2
通讯作者：
Naes,L
中科院分区：
文献类型：
--
作者：
Westfall,TC;Han,SP;Chen,XL;DelValle,K;Curfman,M;Ciarleglio,A;Naes,L
方法在不同品系的大鼠身上进行实验，包括自发性高血压(SHR)、Wistar京都(WKY)(Taconic Farm，Germantown，New Y.)和斯普拉格·道利(SD)(密苏里州圣路易斯萨斯科)。动物在标准的实验室条件下饲养，随意提供食物和水。在实验前1~2天用尾袖体积描记术监测清醒大鼠的血压。使用灌流的肠系膜动脉床，简单地说，从大鼠分离肠系膜血管床，用改良的Krcbs碳酸氢盐缓冲液(含95%)0和5%CO，在37℃下灌流(5ml/分钟)和灌流(0.5ml/分钟)，实验前在肠系膜动脉周围放置一对铂电极用于动脉周围神经刺激(不同频率，2mscs持续最大电压)，允许制剂在实验前平衡45-60分钟。灌流压力由连接到图表记录器的压力传感器连续监测。在一些实验中，灌流液流出液分成1分钟收集到含有0.1N高氯酸的试管中，经氧化铝层析浓缩，经高效液相色谱(HPLC)分离，并通过电化学检测(EC)进行定量。
METHODSExpcriments were carried out in various strains of rats including spontancously hypertensive (SHR), Wistar Kyoto (WKY)(Taconic Farms, Germantown, N. Y.) and Sprague Dawley (SD)(Sasco, St. Louis, Mo.). Animals were maintained under standard laboratory conditions with food and water ad libitum. Blood pressure was monitored in conscious rats by tail cuff plethysmography one or two days prior to experimentation.The perfused mesenteric arterial bed was utilized as described by us prcviously.’Briefly, the mesenteric vascular bed was dissccted from the rat, perfused (5 ml/minute) and superfused (0.5 ml/minute) with modified Krcbs bicarbonate buffer bubblcd with 95%) 0, and 5% CO, at 37 C. A pair of platinum electrodes were placed around the mesenteric artery for periarterial nerve stimulation (various frequencies, 2 mscconds duration supramaximal voltage), The preparation was allowed to equilibratc for 45-60 minutes prior to experimentation. Perfusion pressure was continuously monitored by pressure transducers coupled to chart recorders. In some experiments perfusate effluents were collected in 1-minute fractions into tubes containing 0.1 N perchloric acid, concentrated by alumina chromatography, separated by high performance liquid chromatography (HPLC) and quantified by electrochemical (EC) detection.