Selection of streptavidin binders firom a DNA-encoded chemical library
Selection of streptavidin binders firom a DNA-encoded chemical library
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DOI：
10.1021/bc050282y
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发表时间：
2006-03-01
影响因子：
4.7
通讯作者：
Neri, D
中科院分区：
文献类型：
--
作者：
Dumelin, CE;Scheuermann, J;Neri, D
DNA 编码的小有机分子文库有助于构建大型编码自组装化学文库，用于鉴定与蛋白质靶标的高亲和力结合物。我们构建了一个包含 477 种化合物的文库，与 48 聚体寡核苷酸偶联，每种化合物都包含一个独特的六碱基序列，用作识别化学部分的“条形码”。通过选择和扩增链霉亲和素特异性的高亲和力和低亲和力结合分子，证实了文库的功能。
DNA-encoded libraries of small organic molecules facilitate the construction of large, encoded self-assembling chemical libraries for the identification of high-affinity binders to protein targets. We have constructed a library of 477 chemical compounds, coupled to 48mer-oligonucleotides, each containing a unique six-base sequence serving as "bar-code" for the identification of the chemical moiety. The functionality of the library was confirmed by selection and amplification of both high- and low-affinity binding molecules specific to streptavidin.