Beneficial effects of Brazilian propolis on type 2 diabetes in ob/ob mice: Possible involvement of immune cells in mesenteric adipose tissue.
Beneficial effects of Brazilian propolis on type 2 diabetes in ob/ob mice: Possible involvement of immune cells in mesenteric adipose tissue.
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DOI：
10.4161/adip.25608
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发表时间：
2013-10-01
期刊：
影响因子：
3.3
通讯作者：
Miyoshi I
中科院分区：
文献类型：
--
作者：
Kitamura H;Naoe Y;Kimura S;Miyamoto T;Okamoto S;Toda C;Shimamoto Y;Iwanaga T;Miyoshi I
使用ob/ob小鼠检查巴西甘草的抗糖尿病作用。虽然反复注射巴西枸杞的乙醇提取物（100 mg/kg，ip，每周两次，持续12周）不影响ob/ob小鼠的体重增加和食物摄入，但血糖和血浆胆固醇水平显著降低。此外，黄芪提取物部分恢复葡萄糖耐量和胰岛素抵抗，表明提取物的抗糖尿病特性。蜂胶处理的小鼠在肠系膜脂肪组织中表现出较低的体重增加，而腹股沟和附睾脂肪组织中的体重增加未被调节。流式细胞仪和显微镜分析表明，提取物促进嗜酸性粒细胞积累到肠系膜和附睾脂肪组织。或者，肠系膜脂肪组织中M1样巨噬细胞与M2样巨噬细胞的比例通过注射顺铂降低，与白细胞介素-12A+细胞数量的减少一致。M1巨噬细胞标志物，如Itgax和Il 12 b转录物的水平，降低了肠系膜脂肪组织的血管基质部分，而泛巨噬细胞标志物Emr 1和Cd 68没有受到影响。微阵列和随后的基因本体论术语分析表明，在肠系膜脂肪组织中的免疫激活减弱。综上所述，这表明巴西芦荟改善了ob/ob小鼠的糖尿病，推测是通过修饰肠系膜脂肪组织中的免疫细胞。
The anti-diabetic effects of Brazilian propolis were examined using ob/ob mice. Although repeated injection of an ethanol extract of Brazilian propolis (100 mg/kg, ip, twice a week for 12 weeks) did not affect body weight gain and food intake of ob/ob mice, blood glucose and plasma cholesterol levels were significantly attenuated. Moreover, the propolis extract partially restored glucose tolerance and insulin resistance, indicating anti-diabetic properties of the extract. The propolis-treated mice exhibited lower weight gain in mesenteric adipose tissue, while weight gains in inguinal and epididymal adipose tissues were not modulated. Flow cytometric and microscopic analyses suggested that the extract promoted accumulation of eosinophils into mesenteric and epididymal adipose tissues. Alternatively, the ratio of M1-like macrophages to M2-like macrophages in mesenteric adipose tissue was reduced by the propolis injection, coincident with the decrement of the number of interleukin-12A+ cells. Levels of M1 macrophage markers, such as Itgax and Il12b transcripts, were decreased in the vascular stromal fraction of mesenteric adipose tissue, whereas those of pan-macrophage markers Emr1 and Cd68 were not influenced. Microarray and subsequent gene ontology term analyses suggested that propolis attenuated immune activation in mesenteric adipose tissues. Taken together, this indicates that Brazilian propolis improves diabetes in ob/ob mice, presumably through modification of immune cells in mesenteric adipose tissues.