E-Cadherin negatively modulates delta-catenin-induced morphological changes and RhoA activity reduction by competing with p190RhoGEF for delta-catenin.
E-Cadherin negatively modulates delta-catenin-induced morphological changes and RhoA activity reduction by competing with p190RhoGEF for delta-catenin.
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DOI：
10.1016/j.bbrc.2008.10.030
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发表时间：
2008-12-12
影响因子：
3.1
通讯作者：
Kim, Kwonseop
中科院分区：
文献类型：
--
作者：
Kim, Hangun;Oh, Minsoo;Lu, Qun;Kim, Kwonseop
δ-连环蛋白是犰狳蛋白 p120-连环蛋白亚家族的成员。在这里，我们描述了 HEK293 上皮细胞中 δ-连环蛋白定位的独特特征及其与 E-钙粘蛋白的关联。在维持低细胞密度的 HEK293 细胞中，大约 15% 过度表达 δ-catenin 的细胞显示出树突样过程的形成，但 RhoA 活性没有可检测到的变化。此外，δ-连环蛋白主要位于细胞质中，并与 p190RhoGEF 相关。然而，在高细胞密度下，δ-连环蛋白定位转移到质膜。 δ-catenin 与 E-cadherin 的结合增强，而与 p190RhoGEF 的相互作用减弱。在小鼠胚胎成纤维细胞中，E-钙粘蛋白的异位表达降低了 δ-连环蛋白对 RhoA 活性降低以及树突样过程形成的影响。这些结果表明，δ-连环蛋白更主要地与 E-钙粘蛋白结合，而不是与 p190RhoGEF 结合，并且 δ-连环蛋白的功能依赖于其细胞结合伴侣。
δ-Catenin is a member of the p120-catenin subfamily of armadillo proteins. Here, we describe distinctive features of δ-catenin localization and its association with E-cadherin in HEK293 epithelial cells. In HEK293 cells maintained in low cell densities, approximately 15% of cells overexpressing δ-catenin showed dendrite-like process formation, but there was no detectable change in RhoA activity. In addition, δ-catenin was localized mainly in the cytoplasm and was associated with p190RhoGEF. However, at high cell densities, δ-catenin localization was shifted to the plasma membrane. The association of δ-catenin with E-cadherin was strengthened, whereas its interaction with p190RhoGEF was weakened. In mouse embryonic fibroblast cell, ectopic expression of E-cadherin decreased the effect of δ-catenin on the reduction of RhoA activity as well as on dendrite-like process formation. These results suggest that δ-catenin is more dominantly bound to E-cadherin than to p190RhoGEF, and that δ-catenin’s function is dependent on its cellular binding partner.
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影响因子：
16.2
作者：
Togashi, H;Abe, K;Takeichi, M
通讯作者：
Takeichi, M
DOI：
10.1083/jcb.148.1.189
发表时间：
2000-01-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Thoreson MA;Anastasiadis PZ;Daniel JM;Ireton RC;Wheelock MJ;Johnson KR;Hummingbird DK;Reynolds AB
通讯作者：
Reynolds AB
影响因子：
11.4
作者：
Aberle, H;Bauer, A;Kemler, R
通讯作者：
Kemler, R
影响因子：
6.4
作者：
Burger, MJ;Tebay, MA;Gardiner, RA
通讯作者：
Gardiner, RA
影响因子：
5.4
作者：
Mackie, S;Aitken, A
通讯作者：
Aitken, A