Identification of an S5n allele in Oryza rufipogon Griff. and its effect on embryo sac fertility
Identification of an S5n allele in Oryza rufipogon Griff. and its effect on embryo sac fertility
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DOI：
10.1007/s11434-010-0154-y
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发表时间：
2010-05
影响因子：
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通讯作者：
Chang-Min Wei;Lan Wang;Youxin Yang;Zhixiong Chen;M. Shahid;Jin-quan Li;Xiangdong Liu;Yong-gen Lu
中科院分区：
文献类型：
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作者：
Chang-Min Wei;Lan Wang;Youxin Yang;Zhixiong Chen;M. Shahid;Jin-quan Li;Xiangdong Liu;Yong-gen Lu
水稻具有丰富的遗传多样性，含有许多精英基因。在本作品中，共收录了441篇。利用功能标记s5n对18份材料进行了遗传检测，结果显示该5等位基因为杂合型（S5nS5i/j）。其中14份来自广东省，2份来自广西壮族自治区，1份来自海南省，1份来自江西省。进一步的序列分析跨越了o的ins5等位基因缺失的两侧。rufipogon证实，该等位基因与驯化水稻品种02428的等位基因相同。3种基因型（S5iS5i/S5jS5j:S5nS5i/j:S5nS5n）在15个自交后代中的分离率。rufipogon1显著偏离孟德尔预期（1:2:1），在后代中表现为非杂合型和纯合型的植株比例要低得多。全长5个等位基因的序列分析表明，两种基因间存在少量碱基对差异。rufipogonandO。据推测，s5n可能是一个古老的等位基因。ans5非杂合基因型植株的胚囊育性较低，说明在野生水稻中存在其他控制胚囊育性的基因。
Oryza rufipogonexhibits abundant genetic diversity and contains many elite genes. In this work, a total of 441 accessions ofO. rufipogonwere genetically examined using the functional markerS5n, revealing that 18 accessions carried theS5nallele with a heterozygous genotype (S5nS5i/j). Of these accessions, 14 were from Guangdong Province, 2 from Guangxi Zhuang Autonomous Region, 1 from Hainan Province and 1 from Jiangxi Province. Further sequence analysis spanning both sides of the deletion inS5nallele ofO. rufipogonconfirmed that this allele is identical with that of 02428, a wide-compatibility variety of domesticated rice. The segregation ratio of the 3 genotypes (S5iS5i/S5jS5j:S5nS5i/j:S5nS5n) among 15 self-bred progenies ofO. rufipogonsignificantly deviated from the Mendelian expectation (1:2:1) in that the proportion of plants exhibitingS5nheterozygous and homozygous genotypes was much lower in the progeny. Sequence analysis of full-lengthS5nalleles indicated that a few base pairs were different betweenO. rufipogonandO. sativa, and it was presumed thatS5nmay be an ancient allele. The fertility of the embryo sac in plants with anS5nheterozygous genotype was low, suggesting the existence of other genes controlling embryo sac fertility in the wild rice.