Insecticide Enhancement with Feeding Stimulants in Corn for Western Corn Rootworm Larvae (Coleoptera: Chrysomelidae)
Insecticide Enhancement with Feeding Stimulants in Corn for Western Corn Rootworm Larvae (Coleoptera: Chrysomelidae)
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在玉米中使用饲喂刺激剂增强对西方玉米根虫幼虫的杀虫作用（鞘翅目：叶甲科）
DOI：
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发表时间：
2005
影响因子：
2.2
通讯作者：
L. Bjostad
中科院分区：
文献类型：
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作者：
E. Bernklau;L. Bjostad
摘要 当使用发芽玉米（Zea mays L.）的提取物来引诱新生幼虫取食时，使西方玉米根虫幼虫（Diabrotica virgifera virgifera LeConte）死亡率达到 50% 所需的杀虫剂噻虫嗪的用量减少了 100 倍。在行为生物测定中，新生根虫幼虫在用玉米根压榨液体处理过的滤纸盘上大力进食。此外，用玉米丙酮提取物（干燥并用水再润湿）处理的圆盘也引起了幼虫的强烈摄食。幼虫在不进食的情况下从用蒸馏水处理过的滤纸盘上游走。噻虫嗪的稀释液单独或与玉米提取物一起在生物测定中进行了测试，并且通过添加玉米提取物提高了该杀虫剂的功效。对于含有 10 ppm 噻虫嗪的溶液，当存在玉米提取物时，暴露 30 分钟后，幼虫死亡率为 95%，但在单独测试相同浓度的杀虫剂（不含摄食刺激剂）时，幼虫死亡率仅为 38%。经玉米提取物处理且含有 0.01、0.1、1 或 10 ppm 杀虫剂的圆盘 24 小时后幼虫死亡率显着高于相同浓度但不含玉米提取物的圆盘。即使在测试的最高浓度的噻虫嗪下，噻虫嗪也不会阻止幼虫取食玉米提取物。
Abstract Amounts of the insecticide thiamethoxam required for 50% mortality of western corn rootworm larvae, Diabrotica virgifera virgifera LeConte, were reduced 100-fold when extracts of germinating corn, Zea mays L., were used to entice neonate larvae to feed on it. In behavioral bioassays, neonate rootworm larvae fed vigorously on filter paper disks treated with liquid pressed from corn roots. Moreover, disks treated with an acetone extract of corn (dried and rewetted with water) also elicited strong feeding from larvae. Larvae wandered away from filter paper disks treated with distilled water without feeding. Dilutions of thiamethoxam were tested in the bioassay alone or with corn extract and the efficacy of this insecticide was improved by the addition of the corn extract. For solutions containing 10 ppm thiamethoxam, 95% larval mortality occurred after 30 min of exposure when corn extract was present, but only 38% mortality occurred when the same concentration of insecticide alone (no feeding stimulants) was tested. Larval mortality after 24 h was significantly higher for corn extract-treated disks with 0.01, 0.1, 1, or 10 ppm insecticide than for the same concentrations without corn extract. Thiamethoxam did not deter larval feeding on corn extract, even at the highest concentration of thiamethoxam tested.