Sublethal concentration of emamectin benzoate inhibits the growth of gypsy moth by inducing digestive dysfunction and nutrient metabolism disorder
Sublethal concentration of emamectin benzoate inhibits the growth of gypsy moth by inducing digestive dysfunction and nutrient metabolism disorder
复制标题
亚致死浓度甲维盐通过诱导消化功能障碍和营养代谢障碍抑制舞毒蛾生长
DOI：
10.1002/ps.6432
复制
发表时间：
2021
影响因子：
4.1
通讯作者：
Ma Ling
中科院分区：
文献类型：
--
作者：
Xu Zhe;Bai Jianyang;Li Lu;Liang Liwei;Ma Xiaoqian;Ma Ling
BACKGROUNDGypsy moth (Lymantria dispar) is one of the most important pests in the world. Emamectin benzoate (EMB) is widely used in the control of agricultural and forestry pests. Here, we explored the sublethal effects of EMB on gypsy moths in order to better understand the toxicological mechanism of EMB.RESULTSThe sublethal concentration of EMB exposure significantly decreased the larvae body weight. To further explore the mechanism, indicators related to digestion and nutrient metabolism were detected. The results showed that EMB exposure caused midgut damage, reduced the activities of digestive enzymes and changed the content of sugar and amino acids. Moreover, the expression of insulin/phosphoinositide‐3‐kinase (PI3K)/forkhead box protein O (FoxO) pathway and sugar metabolism‐related genes was abnormal. The expression of insulin receptor (InR), chico, PI3K, and protein kinase B (Akt) significantly reduced, and that of phosphatase and tensin homologue (PTEN) and FoxO increased. The expression of glycogen phosphorylase (GP) was upregulation and that of glycogen synthase (GS), trehalase (TRE) and trehalose‐phosphate synthase (TPS) were downregulation. All results indicated that EMB inhibits the growth of gypsy moth by inducing midgut injury, digestive dysfunction and nutrient metabolism disorder. In addition, EMB caused midgut injury may be related to apoptosis or a collateral effect of the damage in other tissues, and more extensive and deeper research is still needed to investigate the detailed mechanism.CONCLUSIONOur finding strengthens the understanding of the sublethal effect of EMB, and provides a theoretical basis for the application of EMB in the prevention and control of gypsy moth.
