A novel technique of STEM observation of TEM section using LVSEM and application of pathological diagnosis of renal biopsy
A novel technique of STEM observation of TEM section using LVSEM and application of pathological diagnosis of renal biopsy
复制标题
DOI：
10.1093/jmicro/dfac055
复制
发表时间：
2022-10-21
期刊：
影响因子：
1.8
通讯作者：
Takaki, Takashi
中科院分区：
文献类型：
--
作者：
Nagumo, Tasuku;Sakaue, Mari;Takaki, Takashi
透射电子显微镜（TEM）用于病理诊断的有用性是显而易见的。然而，高操作成本和其它缺点限制了维护和操作TEM的能力。近年来，一种价格低廉、操作简便的通用台式低真空扫描电镜（LVSEM）被开发出来，有望应用于电镜病理诊断。到目前为止，我们已经观察了免疫球蛋白A（伊加）肾病的TEM切片与台式LVSEM使用超变压检测器（UVD）和新开发的保持器观察扫描透射电子显微镜（STEM）图像（UVD-STEM保持器），并与典型的TEM观察所获得的图像进行了比较。我们在日本临床分子形态学学会第53届年会和日本显微镜学会第64届研讨会上报告了这一结果，并讨论了这些方法在伊加肾病和其他肾脏疾病病理诊断中的有效性。因此，我们证明了使用台式LVSEM进行病理诊断的潜力。在这项研究中，我们同样检查了典型的肾脏疾病，如膜性肾病，狼疮性肾炎和淀粉样变性。为伊加肾病、膜性肾病和狼疮性肾炎的病理诊断提供了充分的资料。然而，很难检测到淀粉样变性的特征性淀粉样纤维。这种方法的发展有望扩大使用电子显微镜进行病理诊断的可能性，包括其在其他疾病中的应用。
The usefulness of the transmission electron microscope (TEM) for pathological diagnosis is apparent. However, high operating costs and other disadvantages have limited the ability to maintain and operate a TEM. In recent years, a general-purpose benchtop low-vacuum scanning electron microscope (LVSEM), which is inexpensive and easy to operate, has been developed and is expected to be applied in electron microscopic pathological diagnosis. To date, we have previously observed TEM ultrathin sections of Immunoglobulin A (IgA) nephropathy with a benchtop LVSEM using an ultra variable-pressure detector (UVD) and a newly developed holder for observing scanning transmission electron microscope (STEM) images (UVD-STEM holder) and compared the images with those obtained with typical TEM observations. We reported the results in the 53rd Annual Meeting of the Japanese Society for Clinical Molecular Morphology and the 64th Symposium of The Japanese Society of Microscopy and discussed the validity of the methods in the pathological diagnosis of IgA nephropathy and other renal diseases. As a result, we demonstrated the potential for pathological diagnosis using benchtop LVSEM. In this study, we similarly examined typical kidney diseases such as membranous nephropathy, lupus nephritis and amyloidosis. We could obtain sufficient data for the pathological diagnosis of IgA nephropathy, membranous nephropathy and lupus nephritis. However, it is difficult to detect amyloid fibres that are characteristic of amyloidosis. The development of this method is expected to expand the possibilities for pathological diagnosis using electron microscopy, including its application to other diseases.