Generation of three human induced pluripotent stem cell sublines (MZT04D, MZT04J, MZT04C) for reproductive science research
Generation of three human induced pluripotent stem cell sublines (MZT04D, MZT04J, MZT04C) for reproductive science research
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DOI：
10.1016/j.scr.2019.101576
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发表时间：
2019-10-01
影响因子：
1.2
通讯作者：
Clark, Amander T.
中科院分区：
文献类型：
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作者：
Pandolfi, Erica C.;Rojas, Ernesto J.;Clark, Amander T.
我们从人皮肤成纤维细胞（HDF）（MZT 04）产生了三种人诱导多能干细胞（hiPSC）亚系，所述人皮肤成纤维细胞（HDF）（MZT 04）由先前有生育能力的女性捐赠的皮肤活检产生。皮肤活检被广泛同意用于产生用于生物医学研究的hiPSC系，包括专门用于研究人类生育力、不育和生殖细胞的独特同意。使用仙台病毒载体重编程hiPSC，并且随后对自我更新的标志物（包括0 CT 4、NANOG、TRA-1-81和SSEA-4）呈阳性。使用畸胎瘤和PluriTest进一步验证多能性。这些亚系作为hiPSC研究项目的对照，旨在了解女性生育力和不孕症的细胞和分子调节。
We generated three human induced pluripotent stem cell (hiPSC) sublines from human dermal fibroblasts (HDFs) (MZT04) generated from a skin biopsy donated from a previously fertile woman. The skin biopsy was broadly consented for generating hiPSC lines for biomedical research, including unique consent specifically for studying human fertility, infertility and germ cells. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA-4. Pluripotency was further verified using teratomas and PluriTest. These sublines serve as controls for hiPSC research projects aimed at understanding the cell and molecular regulation of female fertility and infertility.