Determination of Flavonoids in Magnolia officinalis Leaves Based on Response Surface Optimization of Infrared Assisted Extraction Followed by High-Performance Liquid Chromatography (HPLC)
Determination of Flavonoids in Magnolia officinalis Leaves Based on Response Surface Optimization of Infrared Assisted Extraction Followed by High-Performance Liquid Chromatography (HPLC)
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基于红外辅助提取响应面优化和高效液相色谱 (HPLC) 测定厚朴叶中的黄酮类化合物
DOI：
10.1080/00032719.2020.1732401
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发表时间：
2020-02-28
影响因子：
2
通讯作者：
Yang, Bin
中科院分区：
文献类型：
--
作者：
Huang, Guoying;Zhang, Mingxiao;Yang, Bin
摘要：采用响应面法和高效液相色谱法，建立了厚朴叶中芦丁、异槲皮苷和槲皮苷的快速提取和测定方法。采用红外辅助提取（IRAE）的Box-Behnken响应面优化法，确定提取黄酮类化合物的最佳工艺条件为：提取时间11 min，乙醇浓度53% (v/v)，液料比40:1，提取功率60 W。与加热回流提取法（HRE）和超声辅助提取法（UAE）相比，IRAE法提取黄酮类化合物的得率分别提高了7.12%和10.59%。此外，rae法提取黄酮类化合物的时间分别约为HRE法和UAE法的1/3和1/9。高效液相色谱法验证了该方法的有效性，并成功地应用于不同地理区域的10种样品的分析。精密度和准确度的相对标准偏差值分别为0.21 ~ 2.33%和0.50 ~ 1.96%，有利于分析应用。回收率达到95%的置信水平。本研究表明，高效液相色谱法是一种简便、经济、可靠的方法，可用于马尾草叶中黄酮类化合物的常规测定。
Abstract A simple and economical method for the rapid extraction and measurement of rutin, isoquercitrin, and quercitrin in Magnolia officinalis leaves was developed using response surface methodology with determination by high performance liquid chromatography (HPLC). By the use of Box-Behnken response surface optimization of infrared-assisted extraction (IRAE), the optimized conditions of flavonoids were as follows: extraction time of 11 min, ethanol concentration of 53% (v/v), liquid–solid ratio of 40:1, and extraction power of 60 W. The yields of flavonoids by the IRAE protocol were higher by 7.12 and 10.59% than using heating reflux extraction (HRE) and ultrasound-assisted extraction (UAE), respectively. In addition, the extraction period of flavonoids by the IRAE method was approximately 1/3 and 1/9 of the time intervals required employing HRE and UAE, respectively. IRAE method was verified by HPLC and successfully applied for analysis of ten samples collected from different geographical areas. The relative standard deviation values of the precision and accuracy were favorable for analytical applications, with values from 0.21 to 2.33% and 0.50 to 1.96%, respectively. The recovery results were achieved at a 95% confidence level. The study shows that the IRAE method coupled with HPLC is a simple, cost-effective and reliable tool for the routine determination of flavonoids in M. officinalis leaves for quality control applications.