MicroRNA-203 leads to G1 phase cell cycle arrest in laryngeal carcinoma cells by directly targeting survivin
MicroRNA-203 leads to G1 phase cell cycle arrest in laryngeal carcinoma cells by directly targeting survivin
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MicroRNA-203 通过直接靶向存活蛋白导致喉癌细胞 G1 期细胞周期停滞。
DOI：
10.1016/j.febslet.2012.01.050
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发表时间：
2012-03-23
期刊：
影响因子：
3.5
通讯作者：
Zhang, Rui
中科院分区：
文献类型：
--
作者：
Bian, Ka;Fan, Jing;Zhang, Rui
先前的研究表明，miR-203在多种癌症中作为肿瘤抑制microRNA发挥作用，但其在喉癌中的作用仍然是矛盾的。在此，我们发现miR-203抑制喉癌细胞的生长，而survivin是miR-203的直接靶点。此外，Survivin的沉默概括了miR-203对细胞周期进程的影响，而Survivin的过表达逆转了这种影响。此外，qRT-PCR结果显示miR-203和survivin在喉癌组织中存在相互关系。这些发现表明miR-203通过直接靶向survivin抑制喉癌细胞的增殖，提示其在抗癌治疗中的应用。(C)2012年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Previous studies have shown that miR-203 acts as a tumor-suppressive microRNA in various cancers, but its roles in laryngeal carcinoma are still contradicted. Here, we found that miR-203 inhibited the growth of laryngeal cancer cells and survivin was a direct target of miR-203. Moreover, silencing of survivin recapitulated the effect of miR-203 on cell cycle progression, whereas overexpression of survivin reversed this effect. Additionally, qRT-PCR showed the reciprocal relationship between miR-203 and survivin in laryngeal cancer tissues. These findings indicate that miR-203 inhibits the proliferation of laryngeal carcinoma cells by directly targeting survivin, suggesting its application in anti-cancer therapeutics. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.