Intestinal microbiome as a risk factor for urinary tract infections in children
Intestinal microbiome as a risk factor for urinary tract infections in children
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DOI：
10.1007/s10096-018-3322-7
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发表时间：
2018-10-01
影响因子：
4.5
通讯作者：
Tapiainen, Terhi
中科院分区：
文献类型：
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作者：
Paalanne, Niko;Husso, Aleksi;Tapiainen, Terhi
由于尿路感染（UTI）病原体来源于肠道，我们假设肠道微生物组反映的肠道环境影响UTI的风险。我们的前瞻性病例对照研究比较了37名发热性UTI儿童与69名健康儿童的肠道微生物组。我们对细菌16 S rRNA基因的区域进行了测序，并使用LefSe算法计算线性判别分析（LDA）效应的大小。我们测量粪便乳铁蛋白和铁浓度和定量PCR大肠杆菌。在门的水平上，没有显着差异。在属水平上，肠杆菌在LDA评分> 3（log 10）的UTI患者中更丰富，而消化链球菌科在LDA评分> 3（log 10）的健康受试者中更丰富。总共观察到20个丰度显著不同的OTU。既往使用抗菌药物与肠道微生物组无关。E. UTI患者和对照组的大肠杆菌感染率分别为1.9%和0.5%（差异的95% CI为0.8 - 3.6%）。定量PCR中大肠杆菌的平均浓度在患者中为0.14 ng/mu l，在对照中为0.08 ng/mu l（差异的95% CI-0.04至0.16）。粪铁和乳铁蛋白浓度组间相似。在家庭和属的水平上，我们注意到UTI儿童和健康儿童之间肠道微生物组的一些差异，这可能意味着肠道环境与儿童UTI的风险有关。
As urinary tract infection (UTI) pathogens originate from the gut, we hypothesized that the gut environment reflected by intestinal microbiome influences the risk of UTI. Our prospective case-control study compared the intestinal microbiomes of 37 children with a febrile UTI with those of 69 healthy children. We sequenced the regions of the bacterial 16S rRNA gene and used the LefSe algorithm to calculate the size of the linear discriminant analysis (LDA) effect. We measured fecal lactoferrin and iron concentrations and quantitative PCR for Escherichia coli. At the phylum level, there were no significant differences. At the genus level, Enterobacter was more abundant in UTI patients with an LDA score > 3 (log 10), while Peptostreptococcaceae were more abundant in healthy subjects with an LDA score > 3 (log 10). In total, 20 OTUs with significantly different abundances were observed. Previous use of antimicrobials did not associate with intestinal microbiome. The relative abundance of E. coli was 1.9% in UTI patients and 0.5% in controls (95% CI of the difference-0.8 to 3.6%). The mean concentration of E.coli in quantitative PCR was 0.14 ng/mu l in the patients and 0.08 ng/mu l in the controls (95% CI of the difference-0.04 to 0.16). Fecal iron and lactoferrin concentrations were similar between the groups. At the family and genus level, we noted several differences in the intestinal microbiome between children with UTI and healthy children, which may imply that the gut environment is linked with the risk of UTI in children.