Infrared heat treatment reduces food intake and modifies expressions of TRPV3-POMC in the dorsal medulla of obesity prone rats.
Infrared heat treatment reduces food intake and modifies expressions of TRPV3-POMC in the dorsal medulla of obesity prone rats.
复制标题
红外热处理可减少食物摄入量，并修饰肥胖容易大鼠背髓质中Trpv3-POMC的表达。
DOI：
10.3109/02656736.2011.601283
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Ma SX
中科院分区：
文献类型：
--
作者：
Hu J;Choo HJ;Ma SX
红外热是一种瞬时受体电位香草酸3型（TRPV3）敏感刺激，可能具有潜在的有益于治疗代谢综合征的生理效应。肥胖倾向（OP）和肥胖抵抗（OR）大鼠以高脂肪饮食喂养7天。热处理的OP大鼠每天暴露于红外光两次，每次20分钟，间隔80分钟的休息。每天测量食物摄入量、血压、血糖和体重，并在治疗的OP大鼠、未治疗的OP大鼠和OR对照之间进行比较。用4%多聚甲醛灌注动物，并用抗TRPV3和前阿黑皮素（POMC）的多克隆抗体在冠状脑干切片上进行免疫组织化学。在显微镜下观察髓质细胞核内的阳性细胞数量。OP大鼠的食物摄入量、体重和平均动脉血压（MAP）较高，OP大鼠是一种饮食诱导的代谢综合征模型，伴有POMC（一种厌食剂）在舌下神经核（HN）和内侧孤束核（mNTS）中的表达减少。热处理OP大鼠的摄食量显著减少。治疗后OP大鼠HN和mNTS的POMC阳性神经元计数增加。在OP对照组大鼠的HN和mNTS中，TRPV3阳性染色神经元增加，而在热处理后减少。HN和mNTS中POMC表达降低和TRPV3表达升高参与了OP大鼠的摄食过多和肥胖的发展。暴露于红外热改变脑干中的TRPV3和POMC表达，减少食物摄入。
Infrared heat, a transient receptor potential vanilloid type-3 (TRPV3) sensitive stimulus, may have potential physiological effects beneficial to treating metabolic syndrome. Obesity prone (OP) and obesity resistant (OR) rats were fed for seven days on a high-fat diet. Heat treated OP rats were exposed twice daily to infrared light for 20 min each, separated by 80 min of rest. Food intake, blood pressure, blood glucose, and body weight measurements were taken daily and compared between treated OP rats, untreated OP rats, and OR controls. The animals were perfused with 4% paraformaldehyde, and immunohistochemistry was performed on the coronal brainstem sections with polyclonal antibodies against TRPV3 and pro-opiomelanocortin (POMC). The positive-staining cells in the medulla nuclei were quantified using a microscope with reticule grid. Food intake, body weight, and mean arterial blood pressure (MAP) were higher in OP rats, a diet-induced metabolic syndrome model, accompanied by a reduced expression of POMC, an anorectic agent, in the hypoglossal nucleus (HN) and medial nucleus tractus solitarius (mNTS). Food intake in heat-treated OP rats was significantly decreased. POMC positive neuron count was increased in the HN and mNTS of OP rats following treatment. TRPV3 positive staining neurons were increased in the HN and mNTS of OP control rats and decreased following the heat treatments. Lowered POMC and heightened TRPV3 expressions in the HN and mNTS are involved in development of hyperphagia and obesity in OP rats. Exposure to infrared heat modifies TRPV3 and POMC expression in the brainstem, reducing food intake.
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影响因子：
5.3
作者：
MAMELI, O;TOLU, E
通讯作者：
TOLU, E
DOI：
10.1152/ajpendo.00451.2006
发表时间：
2007-07-01
影响因子：
5.1
作者：
Li, Gang;Zhang, Yi;Scarpace, Philip J.
通讯作者：
Scarpace, Philip J.
DOI：
10.1523/jneurosci.5741-07.2008
发表时间：
2008-12-17
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Huang SM;Lee H;Chung MK;Park U;Yu YY;Bradshaw HB;Coulombe PA;Walker JM;Caterina MJ
通讯作者：
Caterina MJ
影响因子：
6.9
作者：
Li, Shuyu;Zhang, Hong-Yan;Chen, Yanyun
通讯作者：
Chen, Yanyun
影响因子：
64.8
作者：
Fan, W;Boston, BA;Cone, RD
通讯作者：
Cone, RD