Molecular cloning and characterization of a metal responsive Aedes aegypti intestinal mucin cDNA
Molecular cloning and characterization of a metal responsive Aedes aegypti intestinal mucin cDNA
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DOI：
10.1046/j.1365-2583.2000.00202.x
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发表时间：
2000-08-01
影响因子：
2.6
通讯作者：
Beaty, BJ
中科院分区：
文献类型：
--
作者：
Rayms-Keller, A;McGaw, M;Beaty, BJ
我们从埃及伊蚊中分离出一种 cDNA，该 cDNA 在幼虫中肠中转录以响应金属暴露，并在成年雌性中肠中转录以响应铁或镉暴露或血粉。该 cDNA 编码一种蛋白质，命名为埃及伊蚊肠道粘蛋白 1 (AEIMUC1)，它与无脊椎动物肠道粘蛋白和围营养蛋白以及脊椎动物粘蛋白有相似之处。脯氨酸、丝氨酸和苏氨酸占AEIMUC1氨基酸组成的30%，这是粘蛋白的特征。 AEIMUC1 包含三个富含半胱氨酸的结构域，其中两个位于富含脯氨酸/丝氨酸/苏氨酸的结构域侧翼，这是许多粘蛋白基因共有的特征。这是第一份关于从水生昆虫中分离金属响应基因的报告。
We have isolated a cDNA from Aedes aegypti that is transcribed in the larval midgut in response to metal exposure, and in the adult female midgut in response to iron or cadmium exposure, or a blood meal. The cDNA encodes a protein, designated Aedes aegypti intestinal mucin 1 (AEIMUC1), which has similarities with invertebrate intestinal mucins and peritrophins, and vertebrate mucins. Proline, serine and threonine comprise 30% of the amino acid composition of AEIMUC1, a characteristic of mucins. AEIMUC1 contains three cysteine-rich domains, two of which flank a proline/serine/threonine-rich domain, a feature shared by many mucin genes. This is the first report on the isolation of a metal-responsive gene from an aquatic insect.