Alterations of growth, antioxidant system and gene expression in Stylosanthes guianensis during Colletotrichum gloeosporioides infection.
Alterations of growth, antioxidant system and gene expression in Stylosanthes guianensis during Colletotrichum gloeosporioides infection.
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DOI：
10.1016/j.plaphy.2017.06.024
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发表时间：
2017-09
期刊：
影响因子：
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通讯作者：
Hui Wang;Zhijian Chen;Guodao Liu;C. Bai;H. Qiu;Yan-An Jia;Lijuan Luo
中科院分区：
文献类型：
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作者：
Hui Wang;Zhijian Chen;Guodao Liu;C. Bai;H. Qiu;Yan-An Jia;Lijuan Luo
炭疽病是由炭疽菌（Colletotrichum gloeosporioides）引起的一种最具破坏性的真菌病害，其中包括重要的热带牧草豆科植物柱花草（Stylosanthesspp.）。AlthoughC。孢子虫引起的炭疽病是制约柱头生长和产量的主要病害，目前关于柱头侵染过程中对炭疽病的反应知之甚少。本研究探讨了c对花柱生长、抗氧化系统和基因表达的影响。gloeosporioidestreatment。c .负面影响；接种茎柱的植株出现了球状孢子病，表现为坏死灶的形成和茎部鲜重的减少。茎柱叶片活性氧（ROS）积累增加。gloeosporioidesinfection过程。c处理后，叶片中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）、谷胱甘肽过氧化物酶（GPX）和谷胱甘肽还原酶（GR）等抗氧化酶活性以及抗氧化剂抗坏血酸（AsA）和谷胱甘肽（GSH）浓度均显著升高。gloeosporioidestreatment。此外，转录分析显示，c对胁迫应答基因NADPH氧化酶（Nox）、硫氧还蛋白（Thi）、发病相关基因（pr1和pr5）、苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）、查尔酮合成酶（CHS）和几丁质酶（Cht）的表达有显著增强作用。、。综上所述，本研究提供了关于c感染过程中改变的新信息。在抗氧化系统和基因表达水平上的球茎孢子素。
Anthracnose caused by Colletotrichum gloeosporioides is one of the most destructive fungal diseases of many plants, including stylo (Stylosanthesspp.), which is an important tropical forage legume. AlthoughC. gloeosporioides-caused anthracnose is the major constraint limiting the growth and yield of stylo, little information is available regarding the responses of stylo during the infection process of this pathogen. This study investigated the changes in growth, the antioxidant system and gene expression in stylo in response toC. gloeosporioidestreatment. Negative effects ofC. gloeosporioideswere observed in inoculated stylo plants, as reflected by the formation of necrotic disease lesions and the decrease in shoot fresh weight. Reactive oxygen species (ROS) accumulation increased in stylo leaves during theC.gloeosporioidesinfection process. The activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione peroxidase (GPX) and glutathione reductase (GR), as well as the concentrations of the antioxidant compounds ascorbate (AsA) and glutathione (GSH), increased in leaves underC. gloeosporioidestreatment. Furthermore, transcriptional analysis showed that the expression of stress response genes, including NADPH oxidase (Nox), thioredoxin (Thi),pathogenesis related genes(PR1andPR5), phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), chalcone synthase (CHS) and chitinase (Cht), was differentially enhanced in stylo leaves byC. gloeosporioides. Taken together, this study provides novel information regarding the alterations during the infection process ofC. gloeosporioidesin stylo at the levels of antioxidant system and gene expression.