Long non-coding RNA H19 promotes myoblast fibrogenesis via regulating the miR-20a-5p-Tgfbr2 axis
Long non-coding RNA H19 promotes myoblast fibrogenesis via regulating the miR-20a-5p-Tgfbr2 axis
复制标题
长非编码RNA H19通过调节miR-20a-5p-Tgfbr2轴促进成肌细胞纤维化
DOI：
10.1111/1440-1681.13489
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发表时间：
2021-03-21
影响因子：
2.9
通讯作者：
Chen, Jiwu
中科院分区：
文献类型：
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作者：
Lin, Jinrong;Luo, Zhiwen;Chen, Jiwu
越来越多的证据表明长链非编码RNA（lncRNA）在包括纤维化在内的多种生物学过程中发挥重要作用。在这里，我们报告lncRNA H19能够促进骨骼肌纤维化。lnc-H19被鉴定为在体内和体外骨骼肌纤维化中高度表达;而lnc-H19敲低在体外减弱纤维化。已证明敲低lnc-H19通过海绵状miR-20 a-5 p抑制C2 C12成肌细胞中TGF β/Smad通路的活化，从而通过竞争性内源性RNA功能调节Tgfbr 2表达。我们的研究阐明了lnc-H19-miR-20 a-5 p-Tgfbr 2轴在调节成肌细胞纤维化的TGF β/Smad通路中的作用，这可能为骨骼肌纤维化提供有希望的治疗靶点。
Emerging evidence has indicated long non-coding RNAs (lncRNAs) play important roles in diverse biological processes, including fibrosis. Here, we report that lncRNA H19 is able to promote skeletal muscle fibrosis. lnc-H19 was identified to be highly expressed in skeletal muscle fibrosis in vivo and in vitro; while lnc-H19 knockdown attenuated fibrosis in vitro. The knockdown of lnc-H19 was proved to inhibit the activation of the TGF beta/Smad pathway in C2C12 myoblasts by sponging miR-20a-5p to regulate Tgfbr2 expression through the competing endogenous RNA function. Our study elucidates the roles of the lnc-H19-miR-20a-5p-Tgfbr2 axis in regulating the TGF beta/Smad pathway of myoblast fibrogenesis, which might provide a promising therapeutic target for skeletal muscle fibrosis.