Basophil recruitment and activation in inflammatory skin diseases
Basophil recruitment and activation in inflammatory skin diseases
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DOI：
10.1111/j.1398-9995.2011.02570.x
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发表时间：
2011-08-01
期刊：
影响因子：
12.4
通讯作者：
Yokozeki, H.
中科院分区：
文献类型：
--
作者：
Ito, Y.;Satoh, T.;Yokozeki, H.
背景：嗜碱性粒细胞是血液白细胞，占白细胞的不到1%。它们与肥大细胞具有形态和功能上的相似性，但最近的研究表明，嗜碱性粒细胞通过释放几种细胞因子和脂质介质以及作为抗原呈递细胞发挥非冗余作用。方法：应用碱性粒细胞特异性BB1抗体，对24例皮肤病（136例）组织中嗜碱性粒细胞浸润情况进行免疫组化分析。结果：嗜碱性粒细胞在特应性皮炎、荨麻疹、荨麻疹、大疱性类天疱疮、药疹、嗜酸性脓疱性毛囊炎、虫咬、疥疮、过敏性紫癜、皮肌炎皮损中均有表达。荨麻疹、大疱性类天疱疮和嗜酸性脓疱性毛囊炎的细胞密度显著，而特应性皮炎皮损中的嗜碱性粒细胞数量要少得多。嗜碱性粒细胞在寻常型银屑病、肥大细胞增多症、肿瘤病变、系统性硬化症和系统性红斑狼疮中完全缺失。CD203 c表达水平从荨麻疹和荨麻疹患者的血液嗜碱性粒细胞高于健康donators.Conclusions：嗜碱性粒细胞浸润到皮肤病变比以前认为的更常见，因此，他们可能发挥重要作用，在各种炎症性皮肤疾病。
Background: Basophils are blood leukocytes constituting less than 1% of leukocytes. They share morphological and functional similarities with mast cells, but recent studies indicate that basophils play non-redundant roles via the release of several cytokines and lipid mediators, as well as functioning as antigen presenting cells. However, basophil infiltration into the tissues in human skin diseases remains to be addressed.Methods: The infiltration of basophils in 24 skin diseases (136 samples) was immunohistochemically analyzed using basophil-specific BB1 antibody. In addition, activation of blood basophils was examined by assessing CD203c expression with flow cytometry.Results: Basophils were detected in skin lesions of atopic dermatitis, prurigo, urticaria, bullous pemphigoid, drug eruptions, eosinophilic pustular folliculitis, insect bites, scabies, Henoch-Schonlein purpura and dermatomyositis. While cell densities in urticaria, bullous pemphigoid and eosinophilic pustular folliculitis were prominent, much lower numbers of basophils were seen in lesional skin of atopic dermatitis. Basophils were entirely absent in psoriasis vulgaris, mastocytosis, tumoral lesions, systemic sclerosis, and systemic lupus erythematosus. Levels of CD203c expression on blood basophils from prurigo and urticaria patients were higher than those from healthy donors.Conclusions: Basophils infiltrate into skin lesions more commonly than previously thought, and thus they may play important roles in a variety of inflammatory skin diseases.