Antisense expression of a cell wall-associated protein kinase, WAK4, inhibits cell elongation and alters morphology
Antisense expression of a cell wall-associated protein kinase, WAK4, inhibits cell elongation and alters morphology
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DOI：
10.1105/tpc.13.6.1317
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发表时间：
2001-06-01
期刊：
影响因子：
11.6
通讯作者：
He, ZH
中科院分区：
文献类型：
--
作者：
Lally, D;Ingmire, P;He, ZH
拟南芥细胞壁相关受体样激酶(WAK)基因家族包含5个高度相关的成员，它们的产物适合在细胞内和细胞外之间交换信号。WAK成员在特定的器官中表达，并受各种生物和非生物因素的差异调控。为了进一步了解WAKS在发育过程中的功能，我们使用了糖皮质激素诱导系统异位表达WAK4反义基因。WAK4反义基因的诱导表达导致WAK蛋白表达显著减少。诱导WAK4反义表达96小时后，WAK蛋白检测不到，细胞伸长受损，侧根发育受阻。WAK蛋白的水平可由所用诱导剂地塞米松的浓度控制，并与细胞伸长抑制表型的严重程度相关。这些结果表明，WAKs在细胞伸长中起着至关重要的作用，是植物发育所必需的。
The Arabidopsis cell wall-associated receptor-like kinase (WAK) gene family contains five highly related members whose products are suited for exchanging signals between the intracellular and extracellular compartments. WAK members are expressed in specific organs and regulated differentially by various biotic and abiotic factors. To gain further insight into how WAKs function during development, we used a glucocorticoid-inducible system to express ectopically the WAK4 antisense gene. The induced expression of the WAK4 antisense gene resulted in a significant decrease of WAK proteins. Ninety-six hours after the induction of WAK4 antisense expression, WAK proteins became undetectable, Cell elongation was impaired, and lateral root development was blocked. The level of WAK protein could be controlled by the concentration of the applied inducer, dexamethasone, and was correlated with the severity of the cell elongation inhibition phenotype. These results suggest that the WAKs serve a vital role in cell elongation and are required for plant development.