Establishment and characterization of a human ovarian serous cystadenocarcinoma cell line that produces the tumor markers CA-125 and tissue polypeptide antigen.
Establishment and characterization of a human ovarian serous cystadenocarcinoma cell line that produces the tumor markers CA-125 and tissue polypeptide antigen.
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产生肿瘤标志物 CA-125 和组织多肽抗原的人卵巢浆液性囊腺癌细胞系的建立和表征。
作者：
S. Imai;Y. Kiyozuka;H. Maeda;T. Noda;H. Hosick
从人卵巢浆液性囊腺癌中培养出一株持续产生CA-125和组织多肽抗原（TPA）的细胞（SHIN-3）。1 × 10(5)个细胞培养1周后，观察到高水平的肿瘤标志物（CA-125总释放量为1,500 U， TPA总释放量为37.5 U）。CA-125和TPA在培养细胞中也有表达。每个细胞CA-125的释放量在对数生长期开始前最高。TPA产量在对数生长期有所增加，但与细胞总数的关系尚不清楚。
A cultured cell line (SHIN-3) derived from a human ovarian serous cystadenocarcinoma which consistently produces two tumor markers, CA-125 and tissue polypeptide antigen (TPA) was established. After 1 week of culture of 1 x 10(5) cells, high levels of tumor marker were observed (the total CA-125 release was 1,500 U and the total TPA release was 37.5 U). Expression of CA-125 and TPA was also demonstrated in cultured cells immunohistochemically. The volume of CA-125 release per cell was highest just before the start of the logarithmic growth phase. TPA production was increased in the logarithmic growth phase, but its relationship to the total number of cells was not clear.