Phenanthrene degradation by the bacterium Pseudomonas stutzeri JP1 under low oxygen condition
Phenanthrene degradation by the bacterium Pseudomonas stutzeri JP1 under low oxygen condition
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低氧条件下施氏假单胞菌 JP1 降解菲
DOI：
10.1016/j.ibiod.2017.06.001
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发表时间：
2017
影响因子：
4.8
通讯作者：
Hu Zhong
中科院分区：
文献类型：
--
作者：
Kong Jing;Wang Hui;Liang Lei;Li Lele;Xiong Guangming;Hu Zhong
菲是一种重要的多环芳烃(PAHs)，可以作为多环芳烃的指示剂，也可以作为研究多环芳烃生物降解的模型分子。在菲的生物修复过程中，细菌种类起着重要的作用。本工作以一株已分离的多环芳烃降解菌StutzeriJP1为研究对象，对低氧条件下菲的降解机理进行了研究。RNA测序用于基因检测。结果表明，在PAHs暴露过程中，细菌的甲基巴豆酰-辅酶A羧基酶基因和藜麦蛋白乙醇脱氢酶基因表达上调。RT-qPCR结果表明，藜麦蛋白乙醇脱氢酶编码基因上调了3.16倍。进一步，采用双向电泳法(2-DE)和MALDI-TOF-TOF法对菲降解相关蛋白质进行了研究。结果表明，菲诱导后，6种蛋白质的表达明显增加。藜麦蛋白乙醇脱氢酶基因参与菲的分解代谢。结果与RNA-Seq数据很好地吻合。为低氧环境下微生物修复多环芳烃污染提供了深入的认识和技术支持。
Phenanthrene, is one of the important polycyclic aromatic hydrocarbons (PAHs), which can be used as indicator for measuring PAHs and as a model molecule for studying PAHs biodegradation. During phenanthrene bioremediation bacterial species plays an important. In present work, a previously isolated PAHs-degrading bacterium,Pseudomonas stutzeriJP1, was used to investigate the phenanthrene degradation mechanism under low oxygen condition. RNA-sequencing was conducted for gene detections. The results indicated that methylcrotonoyl-CoA carboxylase gene and quinoprotein alcohol dehydrogenase genes of bacterium were up-regulated during the PAHs exposure. The results of RT-qPCR revealed that the genes encoding the quinoprotein alcohol dehydrogenase were 3.16-fold up-regulated. Further, two-dimensional electrophoresis (2-DE) and MALDI-TOF-TOF were used to study the proteins related to phenanthrene degradation. The results showed that expression of six proteins obviously increased upon phenanthrene induction. Quinoprotein alcohol dehydrogenase gene was involved in phenanthrene catabolism. The results correspond nicely to the RNA-Seq data. This work provided in-depth knowledge as well as technical support for bioremediation of polycyclic aromatic hydrocarbons contamination via microbes in low oxygen environment.