Er:YAG laser promotes proliferation and wound healing capacity of human periodontal ligament fibroblasts through Galectin-7 induction
Er:YAG laser promotes proliferation and wound healing capacity of human periodontal ligament fibroblasts through Galectin-7 induction
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DOI：
10.1016/j.jfma.2020.06.005
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发表时间：
2021-01-01
影响因子：
3.2
通讯作者：
Chen, Chun-Jung
中科院分区：
文献类型：
--
作者：
Lin, Taichen;Yu, Cheng-Chia;Chen, Chun-Jung
背景/目的：在各种牙科激光中，掺铒钇铝石榴石（Er:YAG）激光在合适的能量密度下具有很小的热副作用，在牙周治疗中具有很大的潜力，包括软硬组织消融，并且对组织具有多种作用，有利于伤口愈合。在本研究中，我们试图揭示Er:YAG激光对PDL成纤维细胞影响的分子机制。方法：用不同能量密度（3.6 ~ 6.3 J/cm(2)）的Er:YAG激光照射细胞。MTT法检测细胞增殖，transwell系统检测细胞迁移和侵袭能力。用抓痕法评估伤口愈合能力。在确认这些影响后，应用qRT-PCR和western blotting分析鉴定辐照细胞中半乳糖凝集素-7的差异表达。通过敲低实验揭示半乳糖凝集素-7在Er:YAG激光介导效应中的功能作用。结果：4.2 J/cm(2)为诱导细胞增殖、迁移和侵袭能力的最低能量密度。在上调基因组中，选择半乳糖凝集素-7进一步检测，通过RT-PCR和Western blot验证Er:YAG激光治疗后半乳糖凝集素-7的升高。我们证明了半乳糖凝集素-7的沉默消除了Er:YAG激光对细胞增殖、迁移和侵袭的影响，表明Er:YAG激光通过诱导半乳糖凝集素-7促进了这些作用。结论：Er:YAG激光可通过增强牙周组织的增殖和移动活性来促进牙周组织的再生。此外，半乳糖凝集素-7在Er:YAG激光诱导的益处中的重要性也得到了证实。台湾医学会版权所有2020爱思唯尔台湾有限责任公司出版。
Background/ purpose: Among various dental lasers, the erbium-doped yttrium-aluminumgarnet (Er:YAG) laser has great potential for periodontal treatment including soft and hard tissue ablation with minimal thermal side effects under suitable energy densities and it has multiple effects on tissues for wound-healing benefits. In the present study, we sought to reveal the molecular mechanism underlying the impact of Er:YAG laser on PDL fibroblasts.Methods: Cells were irradiated by a Er:YAG laser with various energy densities (3.6-6.3 J/cm(2)). MTT assay was used for cell proliferation, and the transwell system was employed for migration and invasion abilities. The wound healing capacity was evaluated by a scratch assay. After confirming these effects, qRT-PCR and western blotting analysis was applied to identify the differentially galectin-7 expression in the irradiated cells. Knockdown experiments were conducted to reveal the functional role of galectin-7 in the modulation of Er:YAG laser-mediated effects.Results: 4.2 J/cm(2) was the lowest energy density to induce the optimal cell proliferation, migration and invasion abilities. In the group of upregulated genes, galectin-7 was selected for further examination and its elevation after Er:YAG laser treatment was validated by RT-PCR and Western blot. We demonstrated that silence of galectin-7 abrogated the effects of Er:YAG laser on cell proliferation, migration ad invasion, suggesting the Er:YAG laser promoted these effects through induction of galectin-7.Conclusion: These findings indicated that Er:YAG laser may accelerate the regeneration process in periodontal tissues through enhancement of their proliferative and mobile activities. Additionally, the significance of galectin-7 in the Er:YAG laser-elicited benefits was demonstrated. Copyright (C) 2020, Formosan Medical Association. Published by Elsevier Taiwan LLC.