Catecholamine reinnervation of supraoptic nucleus after deafferenting mechanical lesions and superior cervical ganglionectomy: histofluorescence and functional assessments in young adult and aged rats.
Catecholamine reinnervation of supraoptic nucleus after deafferenting mechanical lesions and superior cervical ganglionectomy: histofluorescence and functional assessments in young adult and aged rats.
复制标题
机械性损伤和颈上神经节切除术后视上核的儿茶酚胺神经支配：年轻成年和老年大鼠的组织荧光和功能评估。
DOI：
10.1016/0006-8993(90)91537-q
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Carlson,SW
中科院分区：
文献类型：
--
作者：
Phelps,CJ;Carlson,SW
Catecholaminergic innervation of the hypothalamic vasopressin (VP)-secreting supraoptic nucleus (SON) was examined at selected intervals after deafferenting neurosurgical lesions, with respect to potential contribution of peripheral vascular sympathetic fibers. Young adult (3 months) and aged (20 months) male F344 rats were subjected to mechanical knife-cut lesion just caudal and medial to the SON, superior cervical ganglionectomy (SCGx), or both surgeries. SON and lesion sites were assessed at 4, 14, 30 or 45 days after surgery, by CA histofluorescence. Functional evaluation in rats subjected to chronic lesions consisted of monitoring water balance (water consumption and urine volumes, and urine osmolality and VP content) in individual rats for presurgical and postsurgical intervals. Histofluorescence evaluation showed that SCGx did not affect the overall SON fluorescence pattern, although a minor sympathetic contribution to that pattern was discerned by comparing SON in rats subjected to lesion alone vs SCGx + lesion. Morphological reinnervation of SON was accomplished at 30 days in young rats, and 45 days in aged rats, after both lesion and SCGx. In young rats, histofluorescence density 30 days after deafferentation was denser than the innervation pattern seen in intact (sham-lesioned) animals, while reinnervation at 45 days postsurgically in aged rats only approximated the presurgical pattern. Vasopressin excretion and corresponding water conservation measures were compromised by SON deafferentation at both ages; excreted VP levels and water balance did not rebound to presurgical values at chronic postsurgical intervals in either young or aged rats. The results indicate that noradrenergic reinnervation of SON after deafferenting lesions may be accomplished in the absence of peripheral sympathetic input, that the phenomenon is intact — although slower and less robust — in aged animals, and that reinnervation at 30 or 45 days after surgery is not accompanied by restoration of presurgical excreted VP levels.
