Role of catalase in metabolism of hydrogen peroxide by the perfused rat heart.
Role of catalase in metabolism of hydrogen peroxide by the perfused rat heart.
复制标题
过氧化氢酶在灌注大鼠心脏过氧化氢代谢中的作用。
DOI：
10.1016/0014-5793(86)80664-0
复制
发表时间：
1986
期刊：
影响因子：
3.5
通讯作者：
Thayer,WS
中科院分区：
文献类型：
--
作者：
Thayer,WS
Cardiac metabolism of H 2 O 2 was studied by determining the concentration dependence for H 2 O 2-stimulated release of GSSG, an indicator for flux through the glutathione peroxidase pathway, in perfused heart preparations. Treatment of rats with aminotriazole in vivo inhibited heart catalase by 83% and shifted the doseresponse curve for GSSG release toward lower H 2 O 2 concentrations. In aminotriazole-treated rats, 50 μM H 2 O 2 elicited a maximal rate of GSSG release (about 5 nmol GSSG min per g heart), whereas 200 μM H 2 O 2 was necessary for obtaining a similar rate of GSSG release in control rat hearts. The results show that catalase, although present at low levels of activity in the heart compared to other organs, functions as a major route for detoxication of H 2 O 2 in the myocardium.
