Peptide-enhanced tumor accumulation of upconversion nanoparticles for sensitive upconversion luminescence/magnetic resonance dual-mode bioimaging of colorectal tumors
Peptide-enhanced tumor accumulation of upconversion nanoparticles for sensitive upconversion luminescence/magnetic resonance dual-mode bioimaging of colorectal tumors
复制标题
肽增强上转换纳米颗粒的肿瘤积累，用于结直肠肿瘤的敏感上转换发光/磁共振双模式生物成像
DOI：
10.1016/j.actbio.2020.01.003
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发表时间：
2020-03-01
影响因子：
9.7
通讯作者：
Zhang, Huimao
中科院分区：
文献类型：
--
作者：
Li, Xinxin;Liu, Lin;Zhang, Huimao
目前，开发高灵敏度、高分辨率的肿瘤靶向纳米颗粒以提高结直肠癌（CRC）的早期无创检测能力仍然是一个巨大的挑战。本研究通过调整核中Er和Yb元素的掺杂比例，制备了在红光区具有高上转换发光（UCL）发射的NaErF4:Yb@NaGdF4:Yb核@壳上转换纳米颗粒（UCNP）。对于生物医学应用，引入羧基封端的二氧化硅壳将所制备的UCNP从有机相转移到水相，并允许分别与源自L-SP5肽的肽配体（即L-SP5-H和L-SP5-C）缀合。由于肽配体中PSP基序的肿瘤靶向亲和力，所制备的肽功能化UCNP（UCNP@SiO2-L-SP5-H和UCNP@SiO2-L-SP5-C）可用作UCL/T1加权磁共振（MR）双模式成像的活性肿瘤靶向造影剂。体外和体内实验结果均表明UCNP@SiO2-L-SP5-C对HCT116 CRC亚型具有较高的亲和力。此外，UCNP@SiO2-L-SP5-C 可以通过体内 UCL 成像可视化超小的皮下异种移植 HCT116 肿瘤（体积约 13 mm(3)）。意义声明1。合成了高红光发射UCNPs用于肿瘤靶向双模式生物成像。2． UCNP@SiO2-L-SP5-C含有肿瘤结合亲和肽，表现出较高的HCT116肿瘤靶向能力。 3． UCNP@SiO2-L-SP5-C成功实现超小HCT116肿瘤的灵敏检测。 (C) 2020 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
Currently, it is still a great challenge to develop tumor targeting nanoparticles with high sensitivity and high resolution for improving the non-invasive detection ability of colorectal cancer (CRC) at an early stage. In this study, NaErF4:Yb@NaGdF4:Yb core@shell upconversion nanoparticles (UCNPs) were prepared with high upconversion luminescence (UCL) emission in red light region through adjusting the doping ratios of Er and Yb elements in the core. For biomedical applications, the carboxyl-terminated silica shell was introduced to transfer the as-prepared UCNPs from the organic phase to the aqueous phase, and allowed conjugation with peptide ligands derived from the L-SP5 peptide (i.e., L-SP5-H and L-SP5-C), respectively. Due to the tumor-targeting affinity of the PSP motif in the peptide ligands, the as-prepared peptide functionalized UCNPs (UCNP@SiO2-L-SP5-H and UCNP@SiO2-L-SP5-C) can be used as an active tumor targeting contrast agents for UCL/T1-weighted magnetic resonance (MR) dual-mode imaging. Both the in vitro and in vivo experimental results demonstrated that UCNP@SiO2-L-SP5-C has relatively high affinity for the HCT116 CRC subtype. Moreover, UCNP@SiO2-L-SP5-C can visualize ultra-small subcutaneous xenografted HCT116 tumors (c.a. 13 mm(3) in volume) by in vivo UCL imaging.Statement of Significance1. High red emission UCNPs were synthesized for tumor-targeting dual-mode bioimaging.2. With tumor-binding affinity peptide, UCNP@SiO2-L-SP5-C shows high HCT116 tumor targeting ability.3. UCNP@SiO2-L-SP5-C successfully achieves sensitive detection of ultrasmall HCT116 tumors. (C) 2020 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.