Phase-randomized laser illumination for microscopy.
Phase-randomized laser illumination for microscopy.
复制标题
用于显微镜的相位随机激光照明。
作者：
R. Hard;R. Zeh;R. Allen
本文介绍了一种简单的装置，它使连续氩离子激光器的输出相位随机化，使它可以用作显微镜的高强度单色光源。相位随机化装置可以与任何激光器（偏振或非偏振的）一起使用，并且具有任何期望的功率输出和波长，包括用于光谱研究的染料激光器。随机化系统可以适用于任何光学显微镜和任何光学系统，包括明场、相衬、Nomarski微分干涉、暗场和分束干涉系统，例如Jamin-Lebedeff系统。它可用于各种测光和摄影研究。514 nm波长似乎对各种细胞相对无害。
A simple apparatus is described that phase randomizes the output of a continuous argon ion laser, so that it may be used as a source of high intensity, monochromatic light for microscopy. The phase-randomizing device can be used with any laser, polarized or unpolarized, and of any desired power output and wavelength, including dye lasers for spectral studies. The randomizing system can be adapted to any light microscope and any optical system including bright-field, phase-contrast, Nomarski differential-interference, dark-field, and split-beam interference systems such as the Jamin-Lebedeff System. It can be used for a variety of photometric and photographic studies. The 514-nm wavelength appears to be relatively harmless to a variety of cells.