Digital autoradiography using room temperature CCD and CMOS imaging technology
Digital autoradiography using room temperature CCD and CMOS imaging technology
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DOI：
10.1088/0031-9155/52/16/019
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发表时间：
2007-08-21
影响因子：
3.5
通讯作者：
Wells, Kevin
中科院分区：
文献类型：
--
作者：
Cabello, Jorge;Bailey, Alexis;Wells, Kevin
CCD（电荷耦合器件）和 CMOS 成像技术可应用于薄组织放射自显影，作为传统胶片的潜在成像替代方案。在这项工作中，我们比较了两种特定的设备：以慢速扫描模式运行的 CCD 和以接近视频速率运行的基于 CMOS 的有源像素传感器。两种成像传感器均在室温下使用直接照射由校准的微尺度和放射性标记的组织样本产生的图像进行操作。我们还将这些数字图像传感器技术与传统胶片的使用进行了比较。我们展示了使用 C-14 校准微尺度和 S-35 放射性标记组织切片获得的比较结果。我们还展示了在室温下运行的 CCD 传感器直接照射下产生的 H-3 图像的第一个结果。与胶片相比，硅基成像技术表现出更高的灵敏度、动态范围和线性度。
CCD (charged coupled device) and CMOS imaging technologies can be applied to thin tissue autoradiography as potential imaging alternatives to using conventional film. In this work, we compare two particular devices: a CCD operating in slow scan mode and a CMOS-based active pixel sensor, operating at near video rates. Both imaging sensors have been operated at room temperature using direct irradiation with images produced from calibrated microscales and radiolabelled tissue samples. We also compare these digital image sensor technologies with the use of conventional film. We show comparative results obtained with C-14 calibrated microscales and S-35 radiolabelled tissue sections. We also present the first results of H-3 images produced under direct irradiation of a CCD sensor operating at room temperature. Compared to film, silicon-based imaging technologies exhibit enhanced sensitivity, dynamic range and linearity.