A comprehensive analysis of three Asiatic black bear mitochondrial genomes (subspecies ussuricus, formosanus and mupinensis), with emphasis on the complete mtDNA sequence of Ursus thibetanus ussuricus (Ursidae)
A comprehensive analysis of three Asiatic black bear mitochondrial genomes (subspecies ussuricus, formosanus and mupinensis), with emphasis on the complete mtDNA sequence of Ursus thibetanus ussuricus (Ursidae)
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DOI：
10.1080/19401730802389525
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发表时间：
2008-01-01
影响因子：
--
通讯作者：
Lee, Jae-Seong
中科院分区：
文献类型：
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作者：
Hwang, Dae-Sik;Ki, Jang-Seu;Lee, Jae-Seong
在本文中，我们描述了亚洲黑熊（Ursus thibetanus ussuricus）的线粒体基因组序列，特别强调控制区（CR），并与线粒体基因组比较熊之间的分子关系。线粒体基因组全序列分析表明，thibetanususussuriicus基因大小为16，700bp，G. 13个蛋白质编码基因，2个rRNA基因，22个tRNA基因）。CR由几个典型的保守结构域（如F、E、D和C盒）和一个保守的序列块组成。不同种的熊CR中的核苷酸序列和重复基序不同，其拷贝数也因种群而异，即使在U.乌苏里虎比较分析表明，CRD1区是高度信息的熊科的歧视。这些结果表明，重复基序和CRD1在熊科的核苷酸序列是很好的物种区分标记。
In the present paper, we describe the mitochondrial genome sequence of the Asiatic black bear (Ursus thibetanus ussuricus) with particular emphasis on the control region (CR), and compared with mitochondrial genomes on molecular relationships among the bears. The mitochondrial genome sequence of U. thibetanus ussuricus was 16,700 bp in size with mostly conserved structures (e. g. 13 protein-coding, two rRNA genes, 22 tRNA genes). The CR consisted of several typical conserved domains such as F, E, D, and C boxes, and a conserved sequence block. Nucleotide sequences and the repeated motifs in the CR were different among the bear species, and their copy numbers were also variable according to populations, even within F1 generations of U. thibetanus ussuricus. Comparative analyses showed that the CR D1 region was highly informative for the discrimination of the bear family. These findings suggest that nucleotide sequences of both repeated motifs and CR D1 in the bear family are good markers for species discriminations.