ARACHIDONIC-ACID METABOLISM BY PLATELETS OF DIFFERING SIZE
ARACHIDONIC-ACID METABOLISM BY PLATELETS OF DIFFERING SIZE
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DOI：
10.1111/j.1365-2141.1983.tb02052.x
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发表时间：
1983-01-01
影响因子：
6.5
通讯作者：
DEYKIN, D
中科院分区：
文献类型：
--
作者：
JAKUBOWSKI, JA;THOMPSON, CB;DEYKIN, D
通过研究不同大小的人血小板对氚标记花生四烯酸（[3 H]AA）的摄取和代谢能力，探讨了血小板平均体积（MPV）与血小板花生四烯酸代谢的关系。用[3 H]AA标记血小板磷脂，然后通过逆流离心将血小板分级成大小依赖性亚群。[3 H]AA的掺入增加通过与MPV成比例的分数。凝血酶刺激后，血小板释放的总~ 3 H放射性百分比随MPV增加而降低。通过HPLC（高效液相色谱法）对释放的3 H-放射性进行分级，结果表明MPV对以环氧合酶产物或HETE（12-羟基二十碳四烯酸）形式释放的总血小板3 H-放射性百分比无显著影响，但未代谢的[3 H]AA的释放随MPV增加而减少。在单独的实验中，使用未标记的血小板，绝对释放的血栓素B2（TXB 2）胶原蛋白和凝血酶诱导的聚集后，通过放射免疫测定法测定，并发现增加成比例的MPV。花生四烯酸代谢产物的释放在不同大小的血小板中显然是定性相似的。血小板掺入花生四烯酸、将其转化为活性代谢物并释放它们的绝对能力与其体积成正比。血小板释放未代谢的花生四烯酸的能力与其MPV成反比。
The relationship between mean platelet volume (MPV) and platelet arachidonic acid metabolism was examined by studying the ability of human platelets of different size of incorporate and metabolize tritiated arachidonic acid ([3H]AA). Platelet phospholipids were labeled with [3H]AA, and the platelets were then fractionated into size-dependent subpopulations by counterflow centrifugation. The incorporation of [3H]AA increased through the fractions proportional to the MPV. After thrombin stimulation the percentage of total 3H-radioactivity released from the platelets decreased as the MPV increased. Fractionation of the released 3H-radioactivity by HPLC (high performance liquid chromatography) demonstrated that MPV had no significant influence on the percentage of total platelet 3H-radioactivity released as cyclooxygenase products or as HETE (12-hydroxyeicosatetraenoic acid), but that the release of unmetabolized [3H]AA decreased as MPV increased. In separate experiments using unlabeled platelets, the absolute release of thromboxane B2 (TXB2) after collagen- and thrombin-induced aggregation was measured by radioimmunoassay and was found to increase in proportion to the MPV. The release of arachidonic acid metabolites apparently is qualitatively similar in platelets of different size. The absolute ability of platelets to incorporate arachidonic acid, convert it to active metabolites and release them is proportional to their volume. The ability of platelets to release unmetabolized arachidonic acid varies inversely with their MPV.