Intermittent Availability of Ethanol Does Not Always Lead to Elevated Drinking in Mice
Intermittent Availability of Ethanol Does Not Always Lead to Elevated Drinking in Mice
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DOI：
10.1093/alcalc/ags067
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发表时间：
2012-09-01
影响因子：
2.8
通讯作者：
Metten, Pamela
中科院分区：
文献类型：
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作者：
Crabbe, John C.;Harkness, John H.;Metten, Pamela
目的：间歇性接触（IA）酒精（乙醇）溶液可导致大鼠摄入比连续接触更高的乙醇，最近的一份报告显示，C57 BL/6 J小鼠每周提供20%乙醇与水3次（先前的研究在24小时内连续或间歇地提供乙醇）。研究方法：我们测试了高偏好的C57 BL/6 J近交系小鼠：我们还研究了黑暗中的高饮酒（HDID）小鼠，这是一种我们选择性繁殖的品系，在短时间内获得一瓶20%的乙醇后达到令人陶醉的血液乙醇水平。结果如下：HDID或C57 BL/6 J雄性小鼠在仅4小时的访问期间每隔一天提供乙醇，其每日摄入量均大于每天提供乙醇4小时的小鼠。在C57 BL/6 J小鼠中，24小时IA的饮水量略有增加。HDID小鼠和它们的对照异质原种的实验紧密地模仿了用C57 BL/6 J小鼠进行的公开研究（Hwa，Chu，Levinson SA等人，Persistent escalation of alcohol drinking in C57 BL/6 J mice with intermittent access to 20% ethanol. Alcohol Clin Exp Res 2011;35：1938-1947）显示，在任何基因型的任一性别中，24小时IA暴露均未显著升高。最后，Hwa等人研究的近似复制显示C57 BL/6 J小鼠的摄入量适度增加，证实了24 h IA的疗效。结论：我们得出结论，4小时的IA可能不足以提高小鼠的饮水量。在HDID小鼠及其对照组中缺乏效果进一步表明，并非所有基因型都对免疫应答有反应。
Aims: Intermittent access (IA) to an alcohol (ethanol) solution can lead rats to higher ethanol intakes than continuous access, and a recent report showed increased drinking in C57BL/6J mice offered 20% ethanol vs. water 3X/week (Prior studies have offered ethanol during 24 h periods, either continuously or intermittently.). Methods: We tested the high-preference C57BL/6J inbred mice: we also studied High Drinking in the Dark (HDID) mice, a line we have selectively bred to reach intoxicating blood ethanol levels after a short period of access to a single bottle of 20% ethanol. Results: Neither HDID or C57BL/6J male mice offered ethanol every other day during only a 4-h access period showed greater daily intake than mice offered ethanol daily for 4 h. There was a small increase in drinking with 24 h IA in C57BL/6J mice. An experiment with HDID mice and their control heterogeneous stock stock modeled closely after a published study with C57BL/6J mice (Hwa, Chu, Levinson SA et al. Persistent escalation of alcohol drinking in C57BL/6J mice with intermittent access to 20% ethanol. Alcohol Clin Exp Res 2011;35:1938-1947) showed no significant elevation with 24 h IA exposure in either sex of any genotype. Finally, a near replication of the Hwa et al. study showed modestly greater intake in C57BL/6J mice, confirming the efficacy of 24 h IA. Conclusion: We conclude that 4 h of IA is likely insufficient to elevate drinking in mice. The lack of effect in HDID mice and their controls further suggests that not all genotypes respond to intermittency.