A mechanism related to the yeast transcriptional regulator Paf1c is required for expression of the Arabidopsis FLC/MAF MADS box gene family
A mechanism related to the yeast transcriptional regulator Paf1c is required for expression of the Arabidopsis FLC/MAF MADS box gene family
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DOI：
10.1105/tpc.104.026062
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发表时间：
2004-11-01
期刊：
影响因子：
11.6
通讯作者：
van Nocker, S
中科院分区：
文献类型：
--
作者：
Oh, S;Zhang, H;van Nocker, S
拟南芥春化独立性（VIP）基因类在发育中具有多种功能，包括通过激活MADSbox基因FLC抑制开花。FLC的表观遗传沉默在通过冷促进开花（vemalization）中起着重要作用。为了更好地了解VIP基因如何影响发育，我们对以前单一特征的VIP 5和VIP 6基因进行了遗传和分子研究。我们发现，这些基因的功能丧失也导致FLC/MAF基因家族的其他成员下调，包括光周期途径调节因子MAF 1/FLM。我们通过定位和转录谱分析克隆了VIP 5和VIP 6。这两种蛋白质与芽殖酵母Paf 1C的不同组分密切相关，Paf 1C是一种转录因子，有助于建立和维持转录促进的染色质修饰，如Bre 1/Rad 6对H2 B的泛素化和三胸相关组蛋白甲基化酶Set 1对组蛋白H3赖氨酸-4的甲基化。遗传分析和免疫共沉淀实验表明，VIP 5和VIP 6的功能与先前描述的VIP 3和VIP 4的机制相同。我们的研究结果表明，进化上保守的转录机制在维持高等真核生物的基因表达中起着至关重要的作用，并在开花中具有核心功能。
The Arabidopsis thaliana VERNALIZATION INDEPENDENCE (VIP) gene class has multiple functions in development, including repression of flowering through activation of the MADSbox gene FLC. Epigenetic silencing of FLC plays a substantial role in the promotion of flowering through cold (vemalization). To better understand how VIP genes influence development, we undertook a genetic and molecular study of the previously unicharacterized VIP5 and VIP6 genes. We found that loss of function of these genes also resulted in downregulation of other members of the FLC/MAF gene family, including the photoperiodic pathway regulator MAF1/FLM. We cloned VIP5 and VIP6 through mapping and transcriptional profiling. Both proteins are closely related to distinct components of budding yeast Paf1C, a transcription factor that assists in establishment and maintenance of transcription-promotive chromatin modifications such as ubiquitination of H2B by Bre1/Rad6 and methylation of histone H3 lysine-4 by the trithorax-related histone methylase Set1. Genetic analysis and coimmunoprecipitation experiments suggest that VIP5 and VIP6 function in the same mechanism as the previously described VIP3 and VIP4. Our findings suggest that an evolutionarily conserved transcriptional mechanism plays an essential role in the maintenance of gene expression in higher eukaryotes and has a central function in flowering.