Human telomerase reverse transcriptase, p53 and Ki-67 expression and apoptosis in colorectal serrated adenoma
Human telomerase reverse transcriptase, p53 and Ki-67 expression and apoptosis in colorectal serrated adenoma
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DOI：
10.1080/003655202760373416
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发表时间：
2002-10-01
影响因子：
1.9
通讯作者：
Chayama, K
中科院分区：
文献类型：
--
作者：
Oka, S;Tanaka, S;Chayama, K
背景：锯齿状腺瘤(SA)最近被认为是结肠直肠的一种独特的组织学病变。然而，目前尚无明确的SA组织病理学标准，其组织发生和自然病程尚不清楚。方法：对25例增生性息肉(HPS)、26例低度恶性息肉(LG-SAS)、32例高度恶性息肉(HG-SAS)、18例低度管状腺瘤(LG-TA)、16例高度恶性腺瘤(HG-TA)和20例原位癌(CIS)进行分析。为了阐明SA的分子特征，我们用原位杂交技术检测了人端粒酶逆转录酶(HTERT)的表达，用免疫组织化学方法检测了P53和Ki-67的表达，用原位DNA缺口末端标记法检测了细胞的凋亡。结果：Hp、LG-SA、HG-SA、LG-TA、HG-TA、CIS的hTERT表达分别为1例(4.0%)、12例(46.2%)、18例(56.3%)、6例(33.3%)、7例(43.8%)、12例(80.0%)。SA组hTERT阳性率明显高于HP组。58例SA中有17例(29%)P53蛋白阳性，但无一例HPS P53免疫反应阳性。SA、TA和CIS的Ki-67标记指数显著高于HP。HP、SA、TA和CIS之间的细胞凋亡指数无显著差异。在HG-SA中，p53阳性病变中hTERT表达的发生率显著高于P53阴性病变。结论：提示SA癌变早期hTERT和P53蛋白表达增强，其恶变过程与TA相似。检测端粒酶逆转录酶和P53蛋白的表达对鉴别诊断SA和Hp有重要意义。
Background: Serrated adenoma (SA) has recently been proposed as a distinct histological lesion of the colorectum. However, no definite histopathologic criteria for SA have been established, and its histogenesis and natural history remain unclear. Methods: We analysed 25 hyperplastic polyps (HPs), 26 low-grade SAs (LG-SAs), 32 high-grade SAs (HG-SAs), 18 low-grade tubular adenomas (LG-TAs), 16 high-grade TAs (HG-TAs) and 20 carcinoma in situ (CIS). To clarify molecular features of SA, we used in situ hybridization to examine the expression of human telomerase reverse transcriptase (hTERT), immunohistochemistry to examine the expressions of p53 and Ki-67, and in situ DNA nick end labeling to detect apoptotic cells. Results: The incidence of hTERT expression was 1 (4.0%) of 25 for HP, 12 (46.2%) of 26 for LG-SA, 18 (56.3%) of 32 for HG-SA, 6 (33.3%) of 18 for LG-TA, 7 (43.8%) of 16 for HG-TA, 12 (80.0%) of 15 for CIS, respectively. The incidence of hTERT expression in SA was significantly higher than that in HP. Seventeen (29%) of the 58 SAs were regarded as positive for p53 protein, but none of the HPs showed p53 immunoreactivity. Ki-67 labeling index in SA, TA and CIS was significantly higher than that in HP. The apoptototic index was not significantly different between HP, SA, TA and CIS. In HG-SA, the incidence of hTERT expression in p53-positive lesions was significantly higher than that in p53-negative lesions. Conclusions: These results suggest that hTERT and p53 expression increase in the early stages of carcinogenesis in SA and that SA has a malignant transformation similar to that of TA. It may be useful to investigate hTERT and p53 expression for differentia l diagnosis of SA from HP.