Novel Pyridinium compound from marine actinomycete, Amycolatopsis alba var. nov DVR D4 showing antimicrobial and cytotoxic activities in vitro
Novel Pyridinium compound from marine actinomycete, Amycolatopsis alba var. nov DVR D4 showing antimicrobial and cytotoxic activities in vitro
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DOI：
10.1016/j.micres.2011.12.003
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发表时间：
2012-01-01
影响因子：
6.7
通讯作者：
Donthireddy, Rami Reddy
中科院分区：
文献类型：
--
作者：
Dasari, Venkata Ratna Ravi Kumar;Muthyala, Murali Krishna Kumar;Donthireddy, Rami Reddy
印度孟加拉湾维沙卡帕特南海岸的海洋沉积物样品作为放线菌的来源进行了调查，以筛选抗生素和细胞毒性化合物的生产。在对海洋放线菌的系统研究中，筛选到一株具有生物活性的放线菌DVR D4。根据生化特性和168 rDNA序列分析，将分离物DVR D4鉴定为白色拟无枝酸菌（Amycolatopsis alba）。溶剂提取，然后进行色谱纯化，有助于分离细胞毒性化合物，根据光谱数据，将其鉴定为1（10-氨基癸基）吡啶盐抗生素。该化合物在体外对宫颈癌（HeLa）、乳腺癌（MCF-7）和脑癌（U87 MG）细胞系显示出有效的细胞毒活性，并且对革兰氏阳性和革兰氏阴性细菌也显示出抗菌活性。(C)2012 Elsevier GmbH. All rights reserved.
Marine sediment samples from Visakhapatnam coast of Bay of Bengal, India, were investigated as a source of actinomycetes to screen for the production of antibiotics and cytotoxic compounds. Actinomycete strain DVR D4 with interesting bioactivity profile was isolated during our systematic study of marine actinomycetes. Based on biochemical properties and 168 rDNA analysis the isolate DVR D4 was identified as a strain of Amycolatopsis alba. A solvent extraction followed by a chromatographic purification helped to isolate a cytotoxic compound, which was identified as 1(10-aminodecyl) Pyridinium salt antibiotic, on the basis of spectral data. The compound showed potent cytotoxic activity against cancer cell lines of cervix (HeLa), breast (MCF-7) and brain (U87MG) in vitro and also exhibited antibacterial activities against Gram-positive and Gram-negative bacteria. (C) 2012 Elsevier GmbH. All rights reserved.