Psychrobacter fulvigenes sp. nov., isolated from a marine crustacean from the Sea of Japan.
Psychrobacter fulvigenes sp. nov., isolated from a marine crustacean from the Sea of Japan.
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黄褐色嗜冷杆菌 sp.
DOI：
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发表时间：
2009
影响因子：
2.8
通讯作者：
V. Mikhailov
中科院分区：
文献类型：
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作者：
L. Romanenko;N. Tanaka;G. Frolova;V. Mikhailov
从日本海采集的海洋甲壳类样本中分离出两种新型类冷杆菌，菌株 KC 40(T) 和 KC 65，并通过多相方法对其进行了表征。根据菌株在常用有机介质上产生黑棕色可扩散颜料的能力来选择菌株，这似乎是冷杆菌属公认成员的独特特征。基于 16S rRNA 和 gyrB 基因序列的系统发育分析表明，新分离株在精神杆菌属内形成了一个单独的簇。菌株 KC 40(T) 和 KC 65 与 Psychrobacter urativorans DSM 14009(T) (98.0 %)、Psychrobacter pulmonis CCUG 46240(T) (97.9 %)、Psychrobacter cibarius JG-219(T) (97.9 %)、粪 Psychrobacter faecalis Iso-46(T) (97.8 %)、Psychrobacter aquimaris SW-210(T) (97.6 %)、Psychrobacter namhaensis SW-242(T) (97.6 %) 和 Psychrobacter nivimaris 88/2-7(T) (97.6 %)。 DNA-DNA 杂交实验显示菌株 KC 40(T) 和 KC 65 之间有 84% 的 DNA-DNA 相关性，但新菌株与已识别的冷杆菌属的模式菌株之间的相关性要低得多 (7-35%)，证实它们属于冷杆菌属的单一新种。根据生理和生化特征的组合，可以将这两种新菌株与已识别的冷杆菌属物种区分开来。根据表型和分子特性，菌株 KC 40(T) 和 KC 65 被认为是 Psychrobacter 属的新种，其名称为 Psychrobacter fulvigenes sp.。十一月被提议。模式菌株是KC 40(T) (=KMM 3954(T)=NRIC 0746(T)=JCM 15525(T))。
Two novel Psychrobacter-like bacteria, strains KC 40(T) and KC 65, were isolated from a marine crustacean specimen collected from the Sea of Japan, and were characterized by using a polyphasic approach. Strains were selected on the basis of their ability to produce black-brown diffusible pigments on commonly used organic media, which appears to be a unique characteristic of recognized members of the genus Psychrobacter. Phylogenetic analyses based on both 16S rRNA and gyrB gene sequences showed that the novel isolates formed a separate cluster within the genus Psychrobacter. Strains KC 40(T) and KC 65 shared highest levels of 16S rRNA gene sequence similarity with Psychrobacter urativorans DSM 14009(T) (98.0 %), Psychrobacter pulmonis CCUG 46240(T) (97.9 %), Psychrobacter cibarius JG-219(T) (97.9 %), Psychrobacter faecalis Iso-46(T) (97.8 %), Psychrobacter aquimaris SW-210(T) (97.6 %), Psychrobacter namhaensis SW-242(T) (97.6 %) and Psychrobacter nivimaris 88/2-7(T) (97.6 %). DNA-DNA hybridization experiments revealed 84 % DNA-DNA relatedness between strains KC 40(T) and KC 65 but much lower levels of relatedness (7-35 %) between the novel strains and the type strains of recognized Psychrobacter species, confirming their assignment to a single novel species of the genus Psychrobacter. The two novel strains could be distinguished from recognized species of the genus Psychrobacter based on a combination of physiological and biochemical characteristics. On the basis of phenotypic and molecular properties, strains KC 40(T) and KC 65 are considered to represent a novel species of the genus Psychrobacter, for which the name Psychrobacter fulvigenes sp. nov. is proposed. The type strain is KC 40(T) (=KMM 3954(T)=NRIC 0746(T)=JCM 15525(T)).