Differential expression of human beta-defensin 2 in keratinized and non-keratinized oral epithelial lesions; immunohistochemistry and in situ hybridization
Differential expression of human beta-defensin 2 in keratinized and non-keratinized oral epithelial lesions; immunohistochemistry and in situ hybridization
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DOI：
10.1007/s004280000303
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发表时间：
2001-03-01
期刊：
影响因子：
3.5
通讯作者：
Kaku, T
中科院分区：
文献类型：
--
作者：
Abiko, Y;Suraweera, AK;Kaku, T
人β-防御素（hBD）-2是由多种上皮细胞产生的一种抗菌肽。由于hBD-2在口腔上皮中的表达尚未评估，我们研究了其在正常口腔上皮和上皮病变中的定位。应用免疫组织化学和原位杂交技术，对30例鳞状细胞癌和6例白斑组织标本中hBD-2的表达进行了研究。hBD-2的免疫染色在过度角化上皮中比在ortho-或nonkeratinized上皮中更强烈。相比之下，hBD-2 mRNA的信号在非角化上皮中经常比在超角化或正角化上皮中更强。结果表明，角化在口腔上皮中起着重要的作用，无论是在mRNA水平上的生物学功能的hBD-2和在上皮中的肽的保留。
Human beta -defensin(hBD)-2, an antimicrobial peptide, is produced by various epithelial cells. Because hBD-2 expression in the oral epithelium has not been assessed, we investigated its localization in normal oral epithelium and epithelial lesions. hBD-2 expression was studied using immunohistochemistry and in situ hybridization on formalin-fixed, paraffin-embedded tissue sections from 30 cases of squamous cell carcinoma and 6 cases of leukoplakia. Immunostaining for hBD-2 was more intense in hyperkeratinized than in ortho- or nonkeratinized epithelium. In contrast, signals for hBD-2 mRNA were frequently stronger in non-keratinized epithelium than in hyper- or ortho-keratinized epithelium. The results suggest that keratinization in oral epithelium plays an important role in the biological function of hBD-2 both at the mRNA level and in the retention of the peptide in the epithelium.