Microdiffusion method for estimation of cyanide in whole blood and its application to the study of conversion of cyanide to thiocyanate.
Microdiffusion method for estimation of cyanide in whole blood and its application to the study of conversion of cyanide to thiocyanate.
复制标题
微扩散法测定全血中氰化物及其在氰化物向硫氰酸盐转化研究中的应用
DOI：
10.1093/clinchem/19.5.466
复制
发表时间：
1973
影响因子：
9.3
通讯作者：
G. Fell
中科院分区：
文献类型：
--
作者：
A. Pettigrew;G. Fell
微扩散技术已被用于分离和浓缩全血中氰化物的纳摩尔量，并允许其简化比色估计(1)。用这种技术测量了正常吸烟者、正常不吸烟者和患有烟草弱视的患者全血中的氰化物。在维生素b12缺乏和正常喂养的Wistar大鼠的全血中也测量了氰化物。从动物实验结果和患者实验结果来看，更合适的氰化物暴露指标可能是血浆硫氰酸盐的改变，而不是直接测定全血氰化物。
A microdiffusion technique has been used to separate and concentrate the nanomole amounts of cyanide in whole blood, and to permit its simplified colorimetric estimation ( 1 ). With this technique cyanide was measured in whole blood from normal smokers, normal nonsmokers, and patients suffering from tobacco amblyopia. Cyanide was also measured in whole blood from vitamin B12-deficient and normally fed Wistar rats. From the experimental animal results and results from patients, it is suggested that a more suitable index of cyanide exposure may be the alteration of plasma thiocyanate rather than the direct determination of whole blood cyanide.