Making and using inexpensive manually constructed tissue micro-array: Experience of a tertiary care hospital in India
Making and using inexpensive manually constructed tissue micro-array: Experience of a tertiary care hospital in India
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DOI：
10.4103/0377-4929.54980
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发表时间：
2009-07-01
影响因子：
1
通讯作者：
Gondal, Ranjana
中科院分区：
文献类型：
--
作者：
Singh, Deepak Kumar;Sakhuja, Puja;Gondal, Ranjana
背景：组织微阵列可以同时分析大量组织。商业阵列仪器的高成本阻碍了这项技术的广泛应用。我们描述了我们的经验，构建组织微阵列在一个简单的方法，使用容易获得和廉价的仪器。材料和方法：我们使用11-19号(G)骨髓环钻活检针/小型有槽螺丝刀在蜡块上打孔。使用骨髓环钻活检针从供体组织块中取出核心，并将其排列到宿主石蜡块中。为每个构造的数组构造一个详细的数据库。结果：该芯片作为免疫组化（IHC）、质控和研究的内部对照，使用时间长达一年。构建一个9点阵列大约需要10分钟，构建一个56点阵列大约需要一个小时。在免疫组化过程中，对照点的平均损失小于1%。即使在4周的储存后，我们也没有发现对照切片的抗原性有任何损失。讨论：本文描述的组织阵列构建技术是自动化仪器的廉价和可靠的替代方案。因为很容易通过改变核心大小来修改阵列，所以很容易根据各个实验室和需求进行调整。我们建议在免疫组化中使用3 × 3至5 × 4网格的核块作为对照，并用于标准化抗体和具有大量核块的阵列块进行研究。
Background: Tissue micro-array enables the analysis of a large number of tissues simultaneously. Widespread use of this technology is hampered by the high cost of commercial array instruments. We describe our experience of constructing tissue micro-array in a simple method using easily available and inexpensive instruments. Materials and Methods: We used an 11-19 gauge (G) bone marrow trephine biopsy needle/ small sized slotted screwdriver to punch holes in the wax blocks. Cores were taken from donor tissue blocks using a bone marrow trephine biopsy needle and arrayed into host paraffin wax blocks. A detailed database was constructed for each array constructed. Results: The array blocks were used over a period of one year as internal control for immunohistochemistry (IHC), quality control and research. It took about 10 minutes to construct a nine-dot array and about one hour for a 56-dot array. During IHC, the average loss of control dots was less than one per cent. We did not see any loss of antigenicity in the control sections even after four weeks storage. Discussion: Tissue array construction by the technique described here is inexpensive and reliable alternative to automated instruments. Because it is easy to modify the arrays by varying the core size, it is easy to adapt this to individual labs and requirements. We recommend using blocks with cores in 3 x 3 to 5 x 4 grids as controls in IHC and for standardizing antibodies and array blocks with a larger number of cores for research.