Overlapping and distinct expression domains of Zic2 and Zic3 during mouse gastrulation
Overlapping and distinct expression domains of Zic2 and Zic3 during mouse gastrulation
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DOI：
10.1016/j.modgep.2004.03.003
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发表时间：
2004-09-01
影响因子：
1.2
通讯作者：
Arkell, R
中科院分区：
文献类型：
--
作者：
Elms, P;Scurry, A;Arkell, R
Zic基因是果蝇奇对基因的脊椎动物同源物。其中两个基因的突变与人类先天性遗传疾病有关。人和小鼠Zic2突变与前脑全畸形有关，前脑全畸形是由前脑腹侧发育缺陷引起的，人和小鼠Zic3突变与左右轴形成失败导致的x连锁异位综合征有关。Zic基因在这些疾病中的胚胎学作用尚不清楚。在这里，我们发现这两个基因在原肠胚形成之前和整个过程中都表达。这些基因在它们的表达域上显示出一些广泛的相似性。然而，这两个基因在一些组织中也有独特的表达，这些独特的结构域与可能在各自遗传疾病的病因学中起作用的区域相关。在原始条纹阶段，Zic2仅在茎节和头突中胚层中短暂表达。颅突是建立或维持腹侧前脑所必需的，而腹侧前脑是前脑无裂畸形中受损的区域。Zic3在原始条纹阶段不表达，但在头褶发育阶段开始在节点内和周围表达。Zic3的这种表达与建立左右轴的第一步密切相关。我们还检测了密切相关的基因Zic1的表达，并没有在原肠胚期胚胎中检测到任何转录本。(C) 2004 Elsevier B.V.版权所有
The Zic genes are the vertebrate homologues of the Drosophila Odd-paired gene. Mutations in two of these genes are associated with human congenital genetic disorders. Mutation of human and mouse Zic2 is associated with holoprosencephaly which is caused by a defect of ventral forebrain development and mutation of human and mouse Zic3 is associated with a X-linked heterotaxy syndrome that results from a failure of left-right axis formation. The embryological role of the Zic genes in these disorders is not well understood. Here we show that both of these genes are expressed prior to and throughout gastrulation. The genes show some broad similarities in their expression domains. Both genes however are also uniquely expressed in some tissues and these unique domains correlate with regions that potentially play a role in the aetiology of the respective genetic disorders. During primitive streak stages Zic2 is expressed transiently and uniquely in the node and the head process mesendoderm. The head process is known to be required for the establishment or maintenance of the ventral forebrain, which is the region disrupted in holoprosencephaly. Zic3 is not expressed in the node during primitive streak stages but is expressed in and around the node beginning from the head fold stages of development. This expression of Zic3 correlates well with the first steps in the establishment of the left-right axis. We also examined the expression of the closely related gene, Zic1, and did not detect any transcripts in gastrulation stage embryos. (C) 2004 Elsevier B.V. All rights reserved.