Overexpression of the CD155 gene in human colorectal carcinoma
Overexpression of the CD155 gene in human colorectal carcinoma
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DOI：
10.1136/gut.49.2.236
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发表时间：
2001-08-01
期刊：
影响因子：
24.5
通讯作者：
Denis, MG
中科院分区：
文献类型：
--
作者：
Masson, D;Jarry, A;Denis, MG
背景和目的-Tage4基因（肿瘤相关糖蛋白E4）在大鼠结肠肿瘤和Min小鼠肠腺瘤中过表达。大鼠Tage 4蛋白与人CD 155具有约40%的同一性，人CD 155是免疫球蛋白超家族的成员，编码能够作为脊髓灰质炎病毒、猪伪狂犬病病毒和牛疱疹病毒1的进入受体的跨膜蛋白。对大鼠Tage4基因的分析揭示了与人CD155基因的结构和功能相似性。因此，我们研究了CD155基因在人类结直肠carcinomas. Methods-整体CD155表达进行了评估，半定量逆转录聚合酶链反应（RT-PCR）和免疫组化分析，使用患者的组织标本结直肠腺瘤和腺癌。我们还使用了一个定性RT-PCR测定，以确定不同的剪接变体在每个sample.Results的相对表达CD155的mRNA水平增加6个结直肠癌组织中的6个肿瘤的自由结肠粘膜相比。免疫组化分析显示，12例CD155蛋白水平升高。定性RT-PCR分析显示，不同的CD155变体转录物的相对表达在不同的正常和癌症样品中是相似的，表明这种过表达与CD155基因选择性剪接产生的特定mRNA变体无关。我们首次发现，CD155基因在结直肠癌中过度表达，这种过度表达开始于肿瘤发生的早期阶段，并持续到晚期。
Background and aims - The Tage4 gene (tumour associated glycoprotein E4) is overexpressed in rat colon tumours and Min mouse intestinal adenomas. The rat Tage4 protein has approximately 40% identity with human CD155, a member of the immunoglobulin superfamily coding for a transmembrane protein capable of serving as an entry receptor for poliovirus, porcine pseudorabies virus, and bovine herpesvirus 1. Analysis of the rat Tage4 gene has revealed structural and functional similarities with the human CD155 gene. We therefore investigated expression of the CD155 gene in human colorectal carcinomas.Methods - Overall CD155 expression was assessed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical analysis using tissue specimens from patients with colorectal adenomas and adenocarcinomas. We also used a qualitative RT-PCR assay to determine relative expression of different splicing variants in each sample.Results - mRNA levels of CD155 were increased in six of six colorectal cancer tissues compared with the tumour free colon mucosa. Immunohistochemical analysis revealed an increased level of CD155 protein in 12 of 12 samples. The qualitative RT-PCR assay revealed that relative expression of the different CD155 variant transcripts was similar in the different normal and cancer samples tested, indicating that this overexpression is not associated with a particular mRNA variant generated by alternative splicing of the CD155 gene.Conclusion - We have shown for the first time that the CD155 gene is overexpressed in colorectal carcinoma and that this overexpression begins at an early stage in tumorigenesis and continues to late stages.