Chemical speciation and association of plutonium with bacteria, kaolinite clay, and their mixture.
Chemical speciation and association of plutonium with bacteria, kaolinite clay, and their mixture.
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DOI：
10.1021/es061207g
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发表时间：
2007-03
影响因子：
11.4
通讯作者：
T. Ohnuki;Takahiro Yoshida;T. Ozaki;N. Kozai;F. Sakamoto;T. Nankawa;Yoshinori Suzuki;A. Francis
中科院分区：
文献类型：
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作者：
T. Ohnuki;Takahiro Yoshida;T. Ozaki;N. Kozai;F. Sakamoto;T. Nankawa;Yoshinori Suzuki;A. Francis
我们调查了Pu（VI）与枯草芽孢杆菌，高岭土粘土，以及两者的混合物的相互作用，以确定和描绘的微生物在调节Pu的环境流动性的作用。将细菌、高岭石及其混合物暴露于pH 5.0的4 x 10（-4）M Pu（VI）溶液中。B吸附钚的量。枯草芽孢杆菌的活性随时间增加，但在48 h内未达到平衡，而高岭石在8 h内达到平衡。B暴露48 h后，溶液中Pu的氧化态明显降低。枯草杆菌和混合物中的Pu-（V），而Pu的氧化态与B有关。枯草芽孢杆菌，混合物为Pu（IV）。从B释放的渗出物。subtilis将Pu（VI）还原为Pu（V）。与此相反，有没有改变在溶液中的Pu的氧化态或高岭石后，暴露于Pu（VI）。扫描电子显微镜-能谱分析表明，混合物中的大部分钚与B缔合。枯草杆菌。这些结果表明，Pu-（IV）优选吸附到混合物中的细菌细胞上，Pu（VI）被还原为Pu（V）和Pu（IV）。
We investigated the interactions of Pu(VI) with Bacillus subtilis, kaolinite clay, and a mixture of the two to determine and delineate the role of the microbes in regulating the environmental mobility of Pu. The bacteria, the kaolinite, and their mixture were exposed to a 4 x 10(-4) M Pu(VI) solution at pH 5.0. The amount of Pu sorbed by B. subtilis increased with time, but had not reached equilibrium in 48 h, whereas equilibrium was attained in kaolinite within 8 h. After 48 h, the oxidation state of Pu in the solutions exposed to B. subtilis and the mixture had changed to Pu-(V), whereas the oxidation state of Pu associated with B. subtilis and the mixture was Pu(IV). Exudates released from B. subtilis reduced Pu(VI) to Pu(V). In contrast, there was no change in the oxidation state of Pu in the solution or on kaolinite after exposure to Pu(VI). Scanning electron microscopy-energy dispersive spectrometry analysis indicated that most of the Pu in the mixture was associated with B. subtilis. These results suggest that Pu-(IV) is preferably sorbed to bacterial cells in the mixture and that Pu(VI) is reduced to Pu(V) and Pu(IV).