Gd-based upconversion nanocarriers with yolk-shell structure for dual-modal imaging and enhanced chemotherapy to overcome multidrug resistance in breast cancer
Gd-based upconversion nanocarriers with yolk-shell structure for dual-modal imaging and enhanced chemotherapy to overcome multidrug resistance in breast cancer
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具有蛋黄壳结构的基于钆的上转换纳米载体用于双模式成像和增强化疗以克服乳腺癌的多药耐药性
DOI：
10.1039/c5nr06522d
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发表时间：
2016-01-01
期刊：
影响因子：
6.7
通讯作者：
Wu, Aiguo
中科院分区：
文献类型：
--
作者：
Pan, Yuanwei;Zhang, Ling'e;Wu, Aiguo
肿瘤的多药耐药（MDR）仍然是一个重大挑战，开发可视化的纳米探针用于耐药肿瘤的诊断和治疗非常重要。在这项工作中，我们开发了一种基于DOX封装的NaYF 4：Yb/Er@NaGdF 4蛋黄壳纳米结构的多功能递送系统，用于耐药乳腺癌的同时双模成像和增强化疗。利用纳米结构的大孔体积，递送系统具有高的装载效率和优异的稳定性。此外，体外和体内毒性研究表明，所制备的蛋黄壳纳米材料具有良好的生物相容性。此外，通过纳米载体递送，可以大大增加耐药MCF-7/ADR细胞对DOX的摄取。与游离DOX相比，该药物载体对MCF-7/ADR细胞的化疗效果明显增强，具有良好的耐多药能力。此外，核壳结构的NaYF4：Yb/Er@NaGdF4提高了上转换发光（UCL）性能，所设计的传输系统也可用于UCL和磁共振（MR）同时成像，是一种很好的双模态成像纳米探针。因此，我们开发了一种多功能的蛋黄壳传递系统，这可能有潜在的应用作为一个可视化的治疗诊断纳米探针，以克服乳腺癌的MDR。
Multidrug resistance (MDR) of cancers is still a major challenge, and it is very important to develop visualized nanoprobes for the diagnosis and treatment of drug resistant cancers. In this work, we developed a multifunctional delivery system based on DOX-encapsulated NaYF4:Yb/Er@NaGdF4 yolk-shell nanostructures for simultaneous dual-modal imaging and enhanced chemotherapy in drug resistant breast cancer. Using the large pore volume of the nanostructure, the delivery system had a high loading efficiency and excellent stability. Also, an in vitro and in vivo toxicity study showed the good biocompatibility of the as-prepared yolk-shell nanomaterials. Moreover, by nanocarrier delivery, the uptake of DOX could be greatly increased in drug resistant MCF-7/ADR cells. Compared with free DOX, the as-prepared delivery system enhanced the chemotherapy efficacy against MCF-7/ADR cells, indicating the excellent capability for overcoming MDR. Furthermore, core-shell NaYF4:Yb/Er@NaGdF4 improved the upconversion luminescence (UCL) performance, and the designed delivery system could also be applied for simultaneous UCL and magnetic resonance (MR) imaging, which could be a good candidate as a dual-modal imaging nanoprobe. Therefore, we developed a multifunctional yolk-shell delivery system, which could have potential applications as a visualized theranostic nanoprobe to overcome MDR in breast cancer.