Kinetic studies on Korean serum cis-AB enzymes reveal diminished A and B transferase activities
Kinetic studies on Korean serum cis-AB enzymes reveal diminished A and B transferase activities
复制标题
DOI：
10.1111/j.1423-0410.2005.00688.x
复制
发表时间：
2005-10-01
期刊：
影响因子：
2.7
通讯作者：
Palcic, MM
中科院分区：
文献类型：
--
作者：
Yazer, MH;Zheng, RB;Palcic, MM
背景顺式AB酶是合成血型A和B抗原的罕见糖基转移酶。我们已经确定了一个大的韩国顺式-AB献血者的队列，并研究了N-乙酰氨基半乳糖转移酶（糖基转移酶A，GTA）和半乳糖基转移酶（糖基转移酶B，GTB）的活性，他们的顺式-AB血清enzymes.Materials和方法的顺式-AB 01等位基因的PCR-RFLP（聚合酶链反应-限制性片段长度多态性）在60个捐助者收集在光州全南红十字会血液中心。结果在具有O等位基因的cis-AB供体中，与正常A和B对照组相比，cis-AB酶的GTA和GTB活性均显著降低（分别为29%和27%）。这与从相应AAAB酶的重组模型的动力学研究预测的行为一致。结论虽然可变，顺式AB酶的功能降低GTA和GTB activity.Summary顺式AB酶的功能可变，但降低GTA和GTB活动与相对较弱的GTB活动，与抗B的正向分型存在的弱凝集反应一致。
Background cis-AB enzymes are rare glycosyltransferases that synthesize both blood group A and B antigens. We have identified a large cohort of Korean cis-AB blood donors and studied the N-acetylgalactosaminyltransferase (glycosyltransferase A, GTA) and galactosyltransferase (glycosyltransferase B, GTB) activity of their cis-AB serum enzymes.Materials and methods The cis-AB01 allele was identified by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) in 60 donors collected at the Gwangju-Chonnam Red Cross Blood Center. Enzyme assays of this cis-AB enzyme were performed on available serum samples from 16 donors with the cis-AB01/O genotype and three with the cis-AB01/A genotype.Results In cis-AB donors with an O allele, both the GTA and GTB activity of the cis-AB enzyme were markedly reduced compared to normal A and B controls (29% and 27%, respectively). This is consistent with the behaviour predicted from kinetic studies of a recombinant model of the corresponding AAAB enzyme.Conclusion Although variable, cis-AB enzymes feature reduced GTA and GTB activities.Summary Cis-AB enzymes feature variable but reduced GTA and GTB activities with relatively weaker GTB activity, consistent with the weak agglutination present on forward typing with anti-B.