Integrating the open field, elevated plus maze and light/dark box to assess different types of emotional behaviors in one single trial
Integrating the open field, elevated plus maze and light/dark box to assess different types of emotional behaviors in one single trial
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DOI：
10.1016/j.bbr.2008.06.007
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发表时间：
2008-11-21
影响因子：
2.7
通讯作者：
Izidio, Geison S.
中科院分区：
文献类型：
--
作者：
Ramos, Andre;Pereira, Elayne;Izidio, Geison S.
目前的焦虑测试并不能单独提供动物情绪状况的纯粹和完整的图像。因此，许多作者使用一系列与焦虑相关的测试来测试他们的实验假设，这些测试被认为反映了情绪的不同方面。本研究的目的是探讨三种广泛使用的行为测试，即开放场（of）、升高加迷宫（EPM）和光/暗盒（LOB），在一次试验中评估更广泛的情绪和非情绪行为的潜在有用性。制定了一种方案，大鼠可以在15分钟内自由探索物理连接到EPM和LDB的OF。每个测试的经典焦虑和运动相关行为都被测量。Lewis和自发性高血压大鼠（SHR）的近交系，在每个单独的测试中都存在遗传差异，在联合装置中，所有与焦虑相关的行为都不同。因素分析显示，三个测试分别或联合应用都会产生类似的焦虑和运动相关因素。在这两种情况下，每个测试都产生了自己的焦虑相关因素。两种苯二氮卓类药物氯二氮环氧化合物（5和10 mg/kg）和咪达唑仑（0.75 mg/kg）促进了对EPM张开臂的接近，而戊四唑（10 mg/kg）特异性抑制了对三个厌恶区域（of中心，EPM张开臂，LDB光室）的探索。总之，这些结果表明，通过提供一种快速、全面和可靠的评估情绪相关行为及其潜在成分的方法，新的集成仪器可能有助于焦虑的研究。(C) 2008年Elsevier B.V.出版
Current anxiety tests do not provide, individually, a pure and complete picture of an animal's emotional profile. Therefore, many authors test their experimental hypotheses using a series of anxiety-related tests, which are thought to reflect different facets of emotionality. The objective of this study was to investigate the potential usefulness of integrating three widely used behavioral tests, the open field (OF), elevated plus maze (EPM), and light/dark box (LOB), to assess a wider range of emotional and non-emotional behaviors within one single trial. A protocol was developed where rats could freely explore an OF that was physically connected to an EPM and a LDB during 15 min. Classical anxiety- and locomotion-related behaviors from each test were measured. Lewis and spontaneously hypertensive rats (SHR) inbred strains, known to present genetic differences in each of the individual tests, differed for all anxiety-related behaviors from the combined apparatus. Factor analyses revealed that similar anxiety- and loco motio n-related factors were produced by the three tests applied either separately or in combination. Under both conditions, each test produced its own anxiety-related factor. Two benzodiazepines, chlordiazepoxide (at 5 and 10 mg/kg) and midazolam (at 0.75 mg/kg), facilitated the approach towards the EPM open arms, whereas pentylenetetrazole (10 mg/kg) specifically inhibited exploration of the three aversive areas (OF center, EPM open arms, LDB light compartment). Together, these results suggest that the new integrated apparatus may contribute to the study of anxiety, by providing a rapid, comprehensive and reliable method of assessing emotionality-related behaviors and its underlying components. (C) 2008 Published by Elsevier B.V.