Biological removal of inhibitors leads to the improved lipid production in the lipid fermentation of corn stover hydrolysate by Trichosporon cutaneum
Biological removal of inhibitors leads to the improved lipid production in the lipid fermentation of corn stover hydrolysate by Trichosporon cutaneum
复制标题
生物去除抑制剂导致皮肤毛孢子菌玉米秸秆水解物脂质发酵过程中脂质产量的提高
DOI：
10.1016/j.biortech.2011.08.024
复制
发表时间：
2011-10-01
影响因子：
11.4
通讯作者：
Bao, Jie
中科院分区：
文献类型：
--
作者：
Huang, Xiao;Wang, Yumei;Bao, Jie
以玉米秸秆(CS)水解液为发酵原料，生产生物柴油潜在原料--皮肤毛孢霉CX1，生产微生物油脂。研究了脂肪发酵的两个主要技术障碍：一是CS预处理过程中产生的木质纤维素降解产物受到强烈抑制；二是CS水解液的碳氮摩尔比(C/N)较低。应用新建立的生物解毒方法去除处理后的CS中的抑制剂。考察了前处理强度和生物解毒强度对油脂发酵的影响。结果表明，生物解毒不仅有效地去除了抑制物，而且降低了氮含量，提高了C/N比。经生物脱毒的CS水解液对T.cutaneum CX1的细胞脂含量达到23.5%，比未脱毒时提高了一倍。(C)2011爱思唯尔有限公司。保留所有权利。
Corn stover (CS) hydrolysate was used as the fermentation feedstock of Trichosporon cutaneum CX1 for production of microbial lipid as the potential raw material of biodiesel. Two major technical barriers of the lipid fermentation were investigated: one was the strong inhibition of lignocellulose degradation compounds generated in the CS pretreatment; the other was the low carbon-to-nitrogen molar ratio (C/N ratio) of the CS hydrolysate. The newly established biodetoxification method was applied to remove the inhibitors in the pretreated CS. The enhancement of the pretreatment severity and the biodetoxification intensity on the lipid fermentation was investigated. The results show that the biodetoxification not only efficiently removed the inhibitor substances, but also led to the reduction of nitrogen content and the increase of C/N ratio. The cell lipid content of T. cutaneum CX1 using the biodetoxified CS hydrolysate reached 23.5%, which was doubled than that using the non-detoxified value. (C) 2011 Elsevier Ltd. All rights reserved.